Martín-Gómez S, Alvarez-Sánchez M A, Rojo-Vázquez F A
Department of Sanidad Animal, Faculty of Veterinary, University of León, C/Profesor Pedro Cármenes s/n, 24071 León, Spain.
J Parasitol. 2005 Jun;91(3):674-8. doi: 10.1645/GE-3423.
Hyperimmune anti-Cryptosporidium parvum ovine colostral whey (HOCW) was tested to determine whether it conferred passive immunity to newborn NMRI mice. Three HOCWs (groups IV-VI), 2 nonimmune colostral wheys (groups II and III), and PBS (group I) were administered once (experiment A) and 3 times (experiment B) daily from -1 to 15 days postinfection (PI). Mice in groups I-VI were inoculated with 5 x 10(5) oocysts (day 0 PI), and group VII mice acted as controls. The percentage and intensity of infection were measured at 6, 9, 12, and 16 days PI. In experiment A, HOCW did not reduce significantly the percentage and intensity of infection except for mice in group VI treated with HOCW with the highest titers of anti-C. parvum antibodies. In contrast, no infection was detected in between 18.7 and 62.5% of the mice in groups IV-VI in experiment B. Furthermore, in these groups, the intensity of the infection decreased significantly, ranging from 83.5 to 97.4%. Thus, HOCW did not completely avoid infection, but a high level of protection was observed, being proportional to the titer of specific antibodies and the amount of whey administered orally. Finally, group VII showed no presence of oocysts.
对超免疫抗微小隐孢子虫绵羊初乳清（HOCW）进行了测试，以确定其是否能赋予新生NMRI小鼠被动免疫力。从感染后（PI）-1天至15天，每天一次（实验A）和每天三次（实验B）给予三种HOCW（IV-VI组）、两种非免疫初乳清（II和III组）以及PBS（I组）。I-VI组小鼠在感染当天（PI第0天）接种5×10⁵个卵囊，VII组小鼠作为对照。在PI第6、9、12和16天测量感染百分比和感染强度。在实验A中，除了用抗微小隐孢子虫抗体滴度最高的HOCW处理的VI组小鼠外，HOCW并未显著降低感染百分比和感染强度。相比之下，在实验B的IV-VI组中，18.7%至62.5%的小鼠未检测到感染。此外，在这些组中，感染强度显著降低，范围为83.5%至97.4%。因此，HOCW并未完全避免感染，但观察到了高水平的保护作用，这与特异性抗体滴度和口服给予的乳清量成正比。最后，VII组未发现卵囊。
