Guitard Juliette, Menotti Jean, Desveaux Allison, Alimardani Parissa, Porcher Raphaël, Derouin Francis, Kapel Nathalie
Laboratoire de Parasitologie-Mycologie, Faculté de Médecine Paris 7, 15 rue de l'Ecole de Médecine, 75006 Paris, France.
Parasitol Res. 2006 Oct;99(5):522-7. doi: 10.1007/s00436-006-0181-4. Epub 2006 Apr 13.
To date, there is no efficient treatment for cryptosporidiosis and parasite eradication relies on innate and acquired immunity. In this study, we investigated the effect of administration of probiotic bacteria on the development and progression of the experimental infection in suckling rats. Rats were fed daily with 2.10(7) CFU of Lactobacillus casei-containing mixture, starting 2 days before the infection until the spontaneous clearance of the parasite. Effects on weight gain, parasite burden, mucosal histology and production of mucosal cytokines (IFNgamma, IL10 and TNFalpha) were studied. Although a trend to a more rapid clearance of parasites was noted in rats treated with probiotics, no significant effect of probiotics administration was observed in terms of weight gain, parasite burden, mucosal damage, or kinetics of mucosal cytokines during the course of infection. Overall, our results showed that the daily administration of L. casei-containing mixtures was unable to eradicate the parasite in our model.
迄今为止，隐孢子虫病尚无有效的治疗方法，寄生虫的根除依赖于先天免疫和后天免疫。在本研究中，我们调查了给乳鼠施用益生菌对实验性感染的发展和进程的影响。从感染前2天开始，每天给大鼠喂食含2×10⁷CFU干酪乳杆菌的混合物，直至寄生虫自然清除。研究了对体重增加、寄生虫负荷、黏膜组织学以及黏膜细胞因子（IFNγ、IL-10和TNFα）产生的影响。尽管在用益生菌治疗的大鼠中观察到寄生虫清除有更快的趋势，但在感染过程中，就体重增加、寄生虫负荷、黏膜损伤或黏膜细胞因子动力学而言，未观察到施用益生菌有显著效果。总体而言，我们的结果表明，在我们的模型中每天施用含干酪乳杆菌的混合物无法根除寄生虫。
