Brígido Marcelo M, Walter Maria Emília M T, Oliveira Adilton G, Inoue Marcus K, Anjos Daniel S, Sandes Edans F O, Gondim João J, Carvalho Maria José de A, Almeida Nalvo F, Felipe Maria Sueli Soares
Department of Cellular Biology, University of Brasília, Brasília, DF, Brazil.
Genet Mol Res. 2005 Jun 30;4(2):203-15.
Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, an endemic mycosis of Latin America. This fungus presents a dimorphic character; it grows as a mycelium at room temperature, but it is isolated as yeast from infected individuals. It is believed that the transition from mycelium to yeast is important for the infective process. The Functional and Differential Genome of Paracoccidioides brasiliensis Project--PbGenome Project was developed to study the infection process by analyzing expressed sequence tags--ESTs, isolated from both mycelial and yeast forms. The PbGenome Project was executed by a consortium that included 70 researchers (professors and students) from two sequencing laboratories of the midwest region of Brazil; this project produced 25,741 ESTs, 19,718 of which with sufficient quality to be analyzed. We describe the computational procedures used to receive process, analyze these ESTs, and help with their functional annotations; we also detail the services that were used for sequence data exploration. Various programs were compared for filtering and grouping the sequences, and they were adapted to a user-friendly interface. This system made the analysis of the differential transcriptome of P. brasiliensis possible.
巴西副球孢子菌是副球孢子菌病的病原体，副球孢子菌病是拉丁美洲的一种地方性真菌病。这种真菌具有双态性；它在室温下以菌丝体形式生长，但从受感染个体中分离出来时是酵母形式。据信，从菌丝体到酵母的转变对感染过程很重要。巴西副球孢子菌功能与差异基因组计划——PbGenome计划的开展是为了通过分析从菌丝体和酵母形式中分离出的表达序列标签（ESTs）来研究感染过程。PbGenome计划由一个联盟执行，该联盟包括来自巴西中西部地区两个测序实验室的70名研究人员（教授和学生）；该项目产生了25741个ESTs，其中19718个质量足以进行分析。我们描述了用于接收、处理、分析这些ESTs并帮助进行功能注释的计算程序；我们还详细介绍了用于序列数据探索的服务。比较了各种程序对序列进行过滤和分组，并使其适应用户友好界面。该系统使得对巴西副球孢子菌差异转录组的分析成为可能。
