Parente Juliana Alves, Costa Milce, Pereira Maristela, Soares Célia Maria de Almeida
Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001-970 Goiânia, GO, Brazil.
Genet Mol Res. 2005 Jun 30;4(2):358-71.
Proteases perform a wide variety of functions inside and outside cells, regulating many biological processes. Infectious microorganisms use proteases, either secreted or attached to their cell surface to weaken and invade their hosts. Therefore, proteases are targets for drugs against a diverse set of diseases. Paracoccidioides brasiliensis is the most prevalent fungal pathogen causing systemic mycosis in Latin America. The development of paracoccidioidomycosis depends on interactions between fungal and host components and proteases have been described as important factors implicated in the mechanism of host colonization by fungi. The primary goal for this study is to present an overview of the transcriptome sequences--identified cDNAs that encode proteases. We obtained a total of 53 cDNAs encoding proteases; 15 were classified as ATP-independent, 12 as ATP-dependent, 22 as proteasome subunits, and 4 as deubiquitinating proteases. The mechanisms and biological activity of these proteases differ in substrate specificity and in catalytic mechanisms.
蛋白酶在细胞内外发挥着多种功能，调节着许多生物过程。传染性微生物利用分泌型或附着于其细胞表面的蛋白酶来削弱并侵入宿主。因此，蛋白酶是针对多种疾病的药物靶点。巴西副球孢子菌是拉丁美洲引起系统性真菌病的最常见真菌病原体。副球孢子菌病的发展取决于真菌与宿主成分之间的相互作用，蛋白酶已被描述为真菌在宿主定植机制中涉及的重要因素。本研究的主要目标是概述转录组序列——已鉴定的编码蛋白酶的cDNA。我们总共获得了53个编码蛋白酶的cDNA；15个被归类为非ATP依赖性，12个为ATP依赖性，22个为蛋白酶体亚基，4个为去泛素化蛋白酶。这些蛋白酶的作用机制和生物活性在底物特异性和催化机制方面有所不同。
