Sato T, Kawamoto A, Tamura A, Tatsumi Y, Fujii T
Department of Biochemistry, Kyoto Pharmaceutical University, Japan.
Chem Pharm Bull (Tokyo). 1992 Mar;40(3):721-4. doi: 10.1248/cpb.40.721.
The antioxidant activities of pueraria glycoside (PG)-1 (isoflavonoid) and mangiferin (xanthonoid) were studied and compared with PG-3 and daidzein (isoflavonoids) and with wogonin (flavonoid). PG-1 and mangiferin rapidly scavenged 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and inhibited lipid peroxidation which was initiated enzymatically by reduced nicotinamide adenine dinucleotide phosphate (NADPH) or non-enzymatically by ascorbic acid or Fenton's reagent (H2O2 + Fe2+) in rat liver microsomes. Wogonin inhibited the enzymatically induced lipid peroxidation but had no scavenging effect on DPPH radical or on the non-enzymatic peroxidation. PG-3 and daidzein did not show any of these effects. Formation of Fe2+ by NADPH-dependent cytochrome P-450 reductase was inhibited by wogonin, but not by PG-1 or mangiferin. PG-1 and mangiferin had no effect on terminating radical chain reaction during the lipid peroxidation in the enzymatic system of microsomes or in the linoleic acid hydroperoxide-induced peroxidation system. These results suggest that PG-1 and mangiferin have an antioxidant activity, probably due to their ability to scavenge free radicals involved in initiation of lipid peroxidation. In contrast, wogonin may affect NADPH-dependent cytochrome P-450 reductase action, since it inhibited only the enzymatically induced lipid peroxidation.
研究了葛根苷（PG）-1（异黄酮）和芒果苷（呫吨酮）的抗氧化活性，并与PG-3、大豆苷元（异黄酮）以及汉黄芩素（黄酮）进行了比较。PG-1和芒果苷能迅速清除1,1-二苯基-2-苦基肼（DPPH）自由基，并抑制脂质过氧化，脂质过氧化可由还原型烟酰胺腺嘌呤二核苷酸磷酸（NADPH）酶促引发，或由抗坏血酸或芬顿试剂（H2O2 + Fe2+）在大鼠肝微粒体中非酶促引发。汉黄芩素抑制酶促诱导的脂质过氧化，但对DPPH自由基或非酶促过氧化没有清除作用。PG-3和大豆苷元没有表现出这些作用。NADPH依赖性细胞色素P-450还原酶形成Fe2+的过程受到汉黄芩素的抑制，但不受PG-1或芒果苷的抑制。在微粒体酶系统或亚油酸氢过氧化物诱导的过氧化系统中，PG-1和芒果苷对脂质过氧化过程中的自由基链反应终止没有影响。这些结果表明，PG-1和芒果苷具有抗氧化活性，可能是由于它们能够清除参与脂质过氧化起始的自由基。相比之下，汉黄芩素可能会影响NADPH依赖性细胞色素P-450还原酶的作用，因为它仅抑制酶促诱导的脂质过氧化。
