从大肠杆菌中提取并通过质谱法鉴定十一异戊烯基二磷酸 - 胞壁酰五肽 - 氨基葡萄糖。
Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli.
作者信息
Guan Ziqiang, Breazeale Steven D, Raetz Christian R H
机构信息
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
出版信息
Anal Biochem. 2005 Oct 15;345(2):336-9. doi: 10.1016/j.ab.2005.07.002.
Undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc (lipid II) is extracted from Escherichia coli cells by utilizing its unusual pH-dependent solubility property in a Bligh-Dyer system, and identified by electrospray ionization mass spectrometry in conjunction with a novel 15N mass shift analysis. The described approach will facilitate the structural characterization of lipid II variants from diverse bacteria, including antibiotic-resistant mutants, as well as the numerous minor uncharacterized lipids present in all biological systems.
通过利用在布莱-戴尔系统中其不寻常的pH依赖性溶解特性，从大肠杆菌细胞中提取十一异戊二烯基二磷酸-MurNAc-五肽-GlcNAc（脂质II），并结合新型15N质量位移分析，通过电喷雾电离质谱法对其进行鉴定。所描述的方法将有助于对来自多种细菌（包括抗生素抗性突变体）的脂质II变体以及所有生物系统中存在的众多未表征的少量脂质进行结构表征。
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