采用醋酸锂/单链载体DNA/聚乙二醇法进行酵母转化。

Yeast transformation by the LiAc/SS Carrier DNA/PEG method.

作者信息

Gietz R Daniel, Woods Robin A

机构信息

Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Canada.

出版信息

Methods Mol Biol. 2006;313:107-20. doi: 10.1385/1-59259-958-3:107.

DOI:10.1385/1-59259-958-3:107

PMID:16118429

Abstract

The technique for the transformation of Saccharomyces cerevisiae using the LiAc/SS Carrier DNA/PEG method is described. We describe a rapid method, for use when large numbers of transformants are not necessary. A high-efficiency method for the generation of large numbers of transformants is also given. A method for the transformation of plasmid libraries, which includes yeast two-hybrid applications, also is listed to aid the reader in generating transformants to effectively cover the library complexity. Finally, a protocol for transformation using a 96-well format is included for transformation applications that require it.

摘要

本文描述了使用醋酸锂/单链载体DNA/聚乙二醇（LiAc/SS Carrier DNA/PEG）方法转化酿酒酵母的技术。我们描述了一种快速方法，适用于不需要大量转化子的情况。还给出了一种用于产生大量转化子的高效方法。列出了一种用于转化质粒文库的方法，其中包括酵母双杂交应用，以帮助读者产生转化子，从而有效覆盖文库的复杂性。最后，针对需要使用96孔板形式进行转化的应用，给出了相应的转化方案。