采用醋酸锂/单链载体DNA/聚乙二醇法进行酵母转化。

Yeast transformation by the LiAc/SS carrier DNA/PEG method.

作者信息

Gietz R Daniel

机构信息

Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Avenue, Winnipeg, MB, Canada, R3E 0W3,

出版信息

Methods Mol Biol. 2014;1163:33-44. doi: 10.1007/978-1-4939-0799-1_4.

DOI:10.1007/978-1-4939-0799-1_4

PMID:24841298

Abstract

Transformation is essential to many molecular and genetic investigations in the yeast Saccharomyces cerevisiae. Yeast transformation protocols utilizing the LiAc/ssDNA/PEG method are presented. Protocols for various applications are listed including a method for transformation in 96-well microtiter plate format and another for the production of frozen competent yeast cells that can be used at a moment's notice.

摘要

转化对于酿酒酵母中的许多分子和遗传学研究至关重要。本文介绍了利用醋酸锂/单链DNA/聚乙二醇方法的酵母转化方案。列出了各种应用的方案，包括96孔微量滴定板形式的转化方法以及另一种用于制备可随时使用的冷冻感受态酵母细胞的方法。