Månsson Per E, Eriksson Carina, Sjödin Kristina
Chemical Ecology, Department of Crop Sciences, Swedish University of Agricultural Sciences, PO Box 44, SE-230 53, Alnarp, Sweden.
J Chem Ecol. 2005 May;31(5):989-1001. doi: 10.1007/s10886-005-4243-3.
Linden (Tilia cordata) bark was shown to contain an antifeedant effective against the large pine weevil, Hylobius abietis. Soxhlet extraction of inner and outer bark resulted in an extract that showed antifeedant activity in a microfeeding assay. The extract was fractionated by chromatography on silica gel using gradient elution with solvents of increasing polarity. The content of the fractions obtained was monitored by thin layer- and gas chromatography. Fractions of similar chemical composition were merged. Two of the 17 fractions showed antifeedant activity in the microfeeding assay. Nonanoic acid was identified in both of these fractions. Subsequent testing in the microfeeding assay showed that nonanoic acid possessed strong antifeedant activity against H. abietis adults.
椴树（心叶椴）树皮被证明含有一种对大型松树象鼻虫（Hylobius abietis）有效的拒食剂。用索氏提取器对内树皮和外树皮进行提取，得到的提取物在微量取食试验中表现出拒食活性。提取物通过硅胶柱色谱法，使用极性递增的溶剂进行梯度洗脱进行分离。通过薄层色谱法和气相色谱法监测所得馏分的含量。化学组成相似的馏分合并在一起。在17个馏分中，有两个在微量取食试验中表现出拒食活性。在这两个馏分中都鉴定出了壬酸。随后在微量取食试验中进行的测试表明，壬酸对松树象鼻虫成虫具有很强的拒食活性。
