Axelsson Karolin, Konstanzer Vera, Rajarao Gunaratna Kuttuva, Terenius Olle, Seriot Lisa, Nordenhem Henrik, Nordlander Göran, Borg-Karlson Anna-Karin
Royal Institute of Technology, School of Chemical Science and Engineering, Department of Chemistry, Ecological Chemistry Group, SE-100 44, Stockholm, Sweden.
Royal Institute of Technology, School of Biotechnology, Division of Microbiology, SE-106 91, Stockholm, Sweden.
Microb Ecol. 2017 Jul;74(1):177-184. doi: 10.1007/s00248-016-0915-5. Epub 2017 Jan 10.
The pine weevil, Hylobius abietis, is a severe forest pest insect as it feeds on newly planted conifer seedlings. To identify and develop an antifeedant could be one step towards the protection of seedlings from feeding damage by the pine weevil. With the aim to trace the origin of the antifeedants previously found in feces of the pine weevil, we investigated the culturable bacteria associated with the gut and identified the volatiles they produced. Bacterial isolates were identified by 16S ribosomal RNA gene analysis. The volatile emissions of selected bacteria, cultivated on NB media or on the grated phloem of Scots pine twigs dispersed in water, were collected and analyzed by solid-phase microextraction gas chromatography-mass spectrometry. The bacterial isolates released a variety of compounds, among others 2-methoxyphenol, 2-phenylethanol, 3-methyl-1-butanol, 1-octen-3-ol, 3-octanone, dimethyl disulfide, and dimethyl trisulfide. A strong antifeedant effect was observed by 2-phenylethanol, which could thus be a good candidate for use to protect planted conifer seedlings against feeding damage caused by H. abietis.
松象甲（Hylobius abietis）是一种严重的森林害虫，它以新种植的针叶树幼苗为食。鉴定和开发一种拒食剂可能是保护幼苗免受松象甲取食危害的重要一步。为了追踪之前在松象甲粪便中发现的拒食剂的来源，我们研究了与肠道相关的可培养细菌，并鉴定了它们产生的挥发性物质。通过16S核糖体RNA基因分析对细菌分离株进行鉴定。收集并通过固相微萃取气相色谱 - 质谱联用仪分析在NB培养基或分散于水中的苏格兰松嫩枝磨碎韧皮部上培养的选定细菌的挥发性排放物。细菌分离株释放出多种化合物，包括2 - 甲氧基苯酚、2 - 苯乙醇、3 - 甲基 - 1 - 丁醇、1 - 辛烯 - 3 - 醇、3 - 辛酮、二甲基二硫和二甲基三硫。观察到2 - 苯乙醇具有很强的拒食作用，因此它可能是用于保护种植的针叶树幼苗免受松象甲取食危害的良好候选物。
