Champness W, Riggle P, Adamidis T, Vandervere P
Department of Microbiology, Michigan State University, East Lansing 48824-1101.
Gene. 1992 Jun 15;115(1-2):55-60. doi: 10.1016/0378-1119(92)90540-6.
To define genetic elements that regulate antibiotic synthesis, we screened for mutations that visibly blocked synthesis of Streptomyces coelicolor's two pigmented antibiotics and found mutant strains in which all four antibiotics were blocked. The responsible mutations defined two loci, absA and absB. Two additional approaches to defining genes have been taken: isolation of cloned genes with a dominant negative effect on antibiotic synthesis and isolation of genes which, in multicopy, can compensate for specific mutational blocks. These genes apparently function in a global regulatory pathway (or network) for control of antibiotic synthesis.
为了确定调控抗生素合成的遗传元件，我们筛选了明显阻断天蓝色链霉菌两种色素抗生素合成的突变，发现了所有四种抗生素合成均被阻断的突变菌株。相关突变确定了两个位点，即absA和absB。我们还采用了另外两种确定基因的方法：分离对抗生素合成具有显性负效应的克隆基因，以及分离多拷贝时可补偿特定突变阻断的基因。这些基因显然在控制抗生素合成的全局调控途径（或网络）中发挥作用。
