Adamidis T, Riggle P, Champness W
Genetics Program, Michigan State University, East Lansing 48824-1101.
J Bacteriol. 1990 Jun;172(6):2962-9. doi: 10.1128/jb.172.6.2962-2969.1990.
Streptomyces coelicolor produces four known antibiotics. To define genetic elements that regulate antibiotic synthesis, we screened for mutations that visibly blocked synthesis of the two pigmented antibiotics and found that the mutant strains which we recovered were of two classes--double mutants and mutants in which all four antibiotics were blocked. The mutations in these multiply blocked strains define a new locus of S. coelicolor which we have named absA. The genetic location of absA, at 10 o'clock, is distinct from the locations of the antibiotic gene clusters and from other known mutations that affect antibiotic synthesis. The phenotype of the absA mutants suggests that all S. coelicolor antibiotic synthesis genes are subject to a common global regulation that is at least in part distinct from sporulation and that absA is a genetic component of the regulatory mechanism.
天蓝色链霉菌能产生四种已知的抗生素。为了确定调控抗生素合成的遗传元件，我们筛选了明显阻断两种有色抗生素合成的突变，结果发现我们获得的突变菌株有两类——双突变体和四种抗生素合成均被阻断的突变体。这些多重阻断菌株中的突变定义了天蓝色链霉菌的一个新基因座，我们将其命名为absA。absA的基因位置在10点钟方向，与抗生素基因簇的位置以及其他影响抗生素合成的已知突变的位置都不同。absA突变体的表型表明，所有天蓝色链霉菌抗生素合成基因都受到一种共同的全局调控，这种调控至少部分有别于孢子形成，而且absA是该调控机制的一个遗传组分。
