Adamidis T, Champness W
Genetics Program, Michigan State University, East Lansing 48824-1101.
J Bacteriol. 1992 Jul;174(14):4622-8. doi: 10.1128/jb.174.14.4622-4628.1992.
The filamentous soil bacterium Streptomyces coelicolor is known to produce four antibiotics which are genetically and structurally distinct. An extensive search for antibiotic regulatory mutants led to the discovery of absB mutants, which are antibiotic deficient but sporulation proficient. Genetic analysis of the absB mutants has resulted in definition of the absB locus at 5 o'clock on the genetic map. Multiple cloned copies of the actII-ORF4 gene, an activator of synthesis of the antibiotic actinorhodin, restore actinorhodin biosynthetic capability to the absB mutants. These results are interpreted to mean that the failure of absB mutants to produce antibiotics results from decreased expression of the antibiotic genes. The absB gene is proposed to be involved in global regulation of antibiotic synthesis.
丝状土壤细菌天蓝色链霉菌已知会产生四种在遗传和结构上不同的抗生素。对抗生素调节突变体进行广泛搜索后发现了absB突变体，这些突变体缺乏抗生素但孢子形成能力正常。对absB突变体的遗传分析已确定absB基因座在遗传图谱上的位置为5点钟方向。抗生素放线紫红素合成的激活剂actII-ORF4基因的多个克隆拷贝可恢复absB突变体的放线紫红素生物合成能力。这些结果被解释为意味着absB突变体无法产生抗生素是由于抗生素基因表达降低所致。有人提出absB基因参与抗生素合成的全局调节。
