Knaut Holger, Blader Patrick, Strähle Uwe, Schier Alexander F
Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, and Department of Cell Biology, New York University School of Medicine, New York, New York 10016, USA.
Neuron. 2005 Sep 1;47(5):653-66. doi: 10.1016/j.neuron.2005.07.014.
Sensory neurons with related functions form ganglia, but how these precisely positioned clusters are assembled has been unclear. Here, we use the zebrafish trigeminal sensory ganglion as a model to address this question. We find that some trigeminal sensory neurons are born at the position where the ganglion is assembled, whereas others are born at a distance and have to migrate against opposing morphogenetic movements to reach the site of ganglion assembly. Loss of Cxcr4b-mediated chemokine signaling results in the formation of mispositioned ganglia. Conversely, ectopic sources of the chemokine SDF1a can attract sensory neurons. Transplantation experiments reveal that neuron-neuron interaction and the adhesion molecules E- and N-Cadherin also contribute to ganglion assembly. These results indicate that ganglion formation depends on the interplay of birthplace, chemokine attraction, cell-cell interaction, and cadherin-mediated adhesion.
具有相关功能的感觉神经元形成神经节，但这些精确定位的细胞簇是如何组装的尚不清楚。在这里，我们以斑马鱼三叉神经感觉神经节为模型来解决这个问题。我们发现，一些三叉神经感觉神经元在神经节组装的位置产生，而其他神经元则在远处产生，并且必须逆着相反的形态发生运动迁移才能到达神经节组装部位。Cxcr4b介导的趋化因子信号缺失会导致错位神经节的形成。相反，趋化因子SDF1a的异位来源可以吸引感觉神经元。移植实验表明，神经元-神经元相互作用以及黏附分子E-钙黏蛋白和N-钙黏蛋白也有助于神经节组装。这些结果表明，神经节的形成取决于出生地、趋化因子吸引、细胞-细胞相互作用以及钙黏蛋白介导的黏附之间的相互作用。
