一种用于检测CD8 +细胞非细胞毒性抗HIV反应的筛查试验。

A screening assay for detecting CD8+ cell non-cytotoxic anti-HIV responses.

作者信息

Killian M Scott, Ng Sharon, Mackewicz Carl E, Levy Jay A

机构信息

AIDS Research Institute and Department of Medicine, University of California San Francisco, San Francisco, CA 94143, United States.

出版信息

J Immunol Methods. 2005 Sep;304(1-2):137-50. doi: 10.1016/j.jim.2005.07.016.

DOI:10.1016/j.jim.2005.07.016

PMID:16129447

Abstract

The rate of HIV-1 disease progression is influenced by several factors that include pathogen and host genetic variations and the quality of antiviral immune responses. The CD8+ cell non-cytotoxic antiviral response (CNAR) substantially suppresses HIV replication in CD4+ cells and is positively associated with an asymptomatic clinical state. Traditionally, the measurement of CNAR has required several culture procedures and costly reagents. Here we report the development and validation of a screening assay for detection of CNAR that accurately identifies individuals benefiting from this response. Use of the CNAR screening assay should facilitate the evaluation of this important immune parameter in studies of HIV pathogenesis, resistance to infection, and vaccine development.

摘要

HIV-1疾病进展速率受多种因素影响，这些因素包括病原体和宿主的基因变异以及抗病毒免疫反应的质量。CD8+细胞非细胞毒性抗病毒反应（CNAR）能显著抑制HIV在CD4+细胞中的复制，且与无症状临床状态呈正相关。传统上，CNAR的检测需要多个培养步骤和昂贵的试剂。在此，我们报告了一种用于检测CNAR的筛查试验的开发与验证，该试验能准确识别受益于这种反应的个体。在HIV发病机制、抗感染和疫苗开发研究中，使用CNAR筛查试验应有助于对这一重要免疫参数的评估。