Ventura Carlo
Laboratory of Molecular Biology and Stem Cell Engineering, National Institute of Biostructures and Biosystems and Institute of Cardiology, S. Orsola-Malpighi Hospital, University of Bologna 40138 Bologna, Italy.
Evid Based Complement Alternat Med. 2005 Sep;2(3):277-83. doi: 10.1093/ecam/neh100. Epub 2005 Jul 20.
Evidence-based medicine is switching from the analysis of single diseases at a time toward an integrated assessment of a diseased person. Complementary and alternative medicine (CAM) offers multiple holistic approaches, including osteopathy, homeopathy, chiropractic, acupuncture, herbal and energy medicine and meditation, all potentially impacting on major human diseases. It is now becoming evident that acupuncture can modify the expression of different endorphin genes and the expression of genes encoding for crucial transcription factors in cellular homeostasis. Extremely low frequency magnetic fields have been found to prime the commitment to a myocardial lineage in mouse embryonic stem cells, suggesting that magnetic energy may direct stem cell differentiation into specific cellular phenotypes without the aid of gene transfer technologies. This finding may pave the way to novel approaches in tissue engineering and regeneration. Different ginseng extracts have been shown to modulate growth and differentiation in pluripotent cells and to exert wound-healing and antitumor effects through opposing activities on the vascular system, prompting the hypothesis that ancient compounds may be the target for new logics in cell therapy. These observations and the subtle entanglement among different CAM systems suggest that CAM modalities may deeply affect both the signaling and transcriptional level of cellular homeostasis. Such a perception holds promises for a new era in CAM, prompting reproducible documentation of biological responses to CAM-related strategies and compounds. To this end, functional genomics and proteomics and the comprehension of the cell signaling networks may substantially contribute to the development of a molecular evidence-based CAM.
循证医学正在从一次分析单一疾病转向对患病个体进行综合评估。补充和替代医学（CAM）提供了多种整体方法，包括整骨疗法、顺势疗法、脊椎按摩疗法、针灸、草药和能量医学以及冥想，所有这些都可能对主要人类疾病产生影响。现在越来越明显的是，针灸可以改变不同内啡肽基因的表达以及细胞内稳态中关键转录因子编码基因的表达。已发现极低频磁场可促使小鼠胚胎干细胞向心肌谱系分化，这表明磁能可能在无需基因转移技术帮助的情况下将干细胞分化为特定的细胞表型。这一发现可能为组织工程和再生的新方法铺平道路。不同的人参提取物已被证明可调节多能细胞的生长和分化，并通过对血管系统的相反作用发挥伤口愈合和抗肿瘤作用，这促使人们提出这样的假设，即古老的化合物可能是细胞治疗新逻辑的靶点。这些观察结果以及不同CAM系统之间的微妙交织表明，CAM模式可能会深刻影响细胞内稳态的信号传导和转录水平。这种认识为CAM的新时代带来了希望，促使人们对与CAM相关的策略和化合物的生物学反应进行可重复的记录。为此，功能基因组学和蛋白质组学以及对细胞信号网络的理解可能会极大地促进基于分子证据的CAM的发展。
