Wan Wu-Qing, Liu Xiao-Yan, Deng Bing
Department of Paediatrics, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2004 Feb;29(1):79-80.
To determine the mechanism of dibutyl phthalate (DBP) to purge leukemic cells by inducing apoptosis.
The effects of DBP on the intracellular free Ca2+ concentration ([Ca2+]i) of leukemic cells were measured by Fura-2AM method. The effects of DBP on the protein expression of c-myc and bcl-2 genes of leukemic cells were measured by immunohistochemical assay.
DBP elicited an intracellular Ca2+ redistribution and a potent extracellular calcium influx in leukemic cells. It also down-regulated the protein expression of c-myc and bcl-2 genes.
DBP can purge leukemic cells in vitro by increasing [Ca2+]i in cells to initiate apoptosis and down-regulate bcl-2 and c-myc proto-gene expression to promote the cell apoptosis.
确定邻苯二甲酸二丁酯（DBP）通过诱导凋亡清除白血病细胞的机制。
采用Fura-2AM法检测DBP对白血病细胞内游离钙离子浓度（[Ca2+]i）的影响。采用免疫组织化学法检测DBP对白血病细胞c-myc和bcl-2基因蛋白表达的影响。
DBP引起白血病细胞内Ca2+重新分布和强大的细胞外钙内流。它还下调了c-myc和bcl-2基因的蛋白表达。
DBP可通过增加细胞内[Ca2+]i启动凋亡，并下调bcl-2和c-myc原癌基因表达促进细胞凋亡，从而在体外清除白血病细胞。
