Chen S P, Tang G C
Department of Hematology, Xiangya Hospital, Central South University, Changsha 410078.
Hunan Yi Ke Da Xue Xue Bao. 2001 Feb 28;26(1):83-5.
This paper was to explore the expressions of bcl-2, c-myc genes and the relationship between them in bone marrow from patients with acute leukemia (AL).
In situ hybridization was used to detect bcl-2 and c-myc mRNA. Immune cytochemistry was used to detect bcl-2 and c-myc protein.
The expressions of bcl-2, c-myc mRNA and proteins were significantly increased in AL; There were not significant differences in bcl-2 mRNA and protein, c-myc mRNA and protein between the patients with acute lymphocytic leukemia and acute nonlymphocytic leukemia; There were linear correlations between bcl-2 mRNA and bcl-2 protein, between c-myc mRNA and c-myc protein, between bcl-2 mRNA and c-myc mRNA, between bcl-2 protein and c-myc protein.
bcl-2 and c-myc genes play an important role in pathogenesis and the development of AL.
探讨急性白血病（AL）患者骨髓中bcl-2、c-myc基因的表达及其相互关系。
采用原位杂交法检测bcl-2和c-myc mRNA，免疫细胞化学法检测bcl-2和c-myc蛋白。
AL患者骨髓中bcl-2、c-myc mRNA及蛋白表达均显著升高；急性淋巴细胞白血病与急性非淋巴细胞白血病患者之间bcl-2 mRNA与蛋白、c-myc mRNA与蛋白表达差异无统计学意义；bcl-2 mRNA与bcl-2蛋白、c-myc mRNA与c-myc蛋白、bcl-2 mRNA与c-myc mRNA、bcl-2蛋白与c-myc蛋白之间均呈线性相关。
bcl-2和c-myc基因在AL的发病机制及病情发展中起重要作用。
