冷凝温度对选定呼气参数的影响。
Influence of condensation temperature on selected exhaled breath parameters.
作者信息
Goldoni Matteo, Caglieri Andrea, Andreoli Roberta, Poli Diana, Manini Paola, Vettori Maria Vittoria, Corradi Massimo, Mutti Antonio
机构信息
National Institute of Occupational Safety and Prevention, Research Center at the University of Parma, Parma, Italy.
出版信息
BMC Pulm Med. 2005 Sep 1;5:10. doi: 10.1186/1471-2466-5-10.
BACKGROUND
The effects of changes in cooling temperature on biomarker levels in exhaled breath condensate have been little investigated. The aim of the study was to test the effect of condensation temperature on the parameters of exhaled breath condensate and the levels of selected biomarkers.
METHODS
Exhaled breath condensate was collected from 24 healthy subjects at temperatures of -10, -5, 0 and +5 C degrees. Selected parameters (condensed volume and conductivity) and biomarkers (hydrogen peroxide, malondialdehyde) were measured.
RESULTS
There was a progressive increase in hydrogen peroxide and malondialdehyde concentrations, and condensate conductivity as the cooling temperature increased; total condensate volume increased as the cooling temperature decreased.
CONCLUSION
The cooling temperature of exhaled breath condensate collection influenced selected biomarkers and potential normalizing factors (particularly conductivity) in different ways ex vivo. The temperature of exhaled breath condensate collection should be controlled and reported.
背景
呼气冷凝物中冷却温度变化对生物标志物水平的影响鲜有研究。本研究的目的是测试冷凝温度对呼气冷凝物参数及所选生物标志物水平的影响。
方法
从24名健康受试者中收集呼气冷凝物，收集温度分别为-10℃、-5℃、0℃和+5℃。测量所选参数（冷凝体积和电导率）及生物标志物（过氧化氢、丙二醛）。
结果
随着冷却温度升高，过氧化氢和丙二醛浓度以及冷凝物电导率逐渐增加；随着冷却温度降低，冷凝物总体积增加。
结论
呼气冷凝物收集的冷却温度在体外以不同方式影响所选生物标志物和潜在的标准化因子（尤其是电导率）。应控制并报告呼气冷凝物收集的温度。
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