Nalik H P, Müller K D, Ansorg R
Institut für Medizinische Mikrobiologie, Universität, (GHS) Essen, Germany.
J Med Microbiol. 1992 Jun;36(6):371-6. doi: 10.1099/00222615-36-6-371.
Transesterification of bacterial fatty acids to methyl esters with trimethylsulphonium hydroxide (TMSH) was compared with a conventional method for the identification of Legionella species by capillary gas chromatography. There was an extensive coincidence in the gas chromatographic profiles of bacterial fatty acid methyl esters (FAMEs) obtained by the two methods. However, the TMSH procedure needs less initial material and is much more simple and rapid. The chromatographic pattern of FAMEs obtained from a single colony is sufficient for the identification of the genus Legionella, and L. pneumophila can be clearly distinguished from other Legionella species.
将细菌脂肪酸与氢氧化三甲基锍（TMSH）进行酯交换反应生成甲酯，并与通过毛细管气相色谱法鉴定军团菌属菌种的传统方法进行比较。两种方法所获得的细菌脂肪酸甲酯（FAMEs）的气相色谱图有广泛的一致性。然而，TMSH方法所需的起始材料较少，而且更加简单快捷。从单个菌落获得的FAMEs的色谱图足以鉴定军团菌属，并且嗜肺军团菌能够与其他军团菌属菌种清楚地区分开来。
