Marmet D, Bornstein N, Fleurette J
Laboratoire National de la Santé, Faculté de Médecine Alexis-Carrel, Lyon.
Ann Biol Clin (Paris). 1988;46(6):371-5.
The fatty acids and ubiquinones of 44 reference strains corresponding to species of Legionella and 3 strains of Legionella isolated in the environment have been evaluated. The analysis of fatty acids profiles allows a classification of 22 species into 4 groups depending on the predominance of some branched-chain fatty acids of linear fatty acids. As for ubiquinones our results for the 22 species corroborate the classification in 5 groups established by Moss in 1983 based on the study of 10 species of Legionella. The analysis of fatty acids composition combined with ubiquinones content has allowed us to identify the antigenically similar strains which cannot be differentiated by direct immunofluorescence on one hand (strain 1) and on the other a more precise approach to the identification, before DNA-ADN hybridization of strains of new species (strains 2).
对与军团菌属各物种相对应的44株参考菌株以及从环境中分离出的3株军团菌的脂肪酸和泛醌进行了评估。脂肪酸谱分析可根据某些支链脂肪酸或直链脂肪酸的优势将22个物种分为4组。至于泛醌，我们对这22个物种的研究结果证实了莫斯于1983年基于对10种军团菌的研究而建立的5组分类。脂肪酸组成分析与泛醌含量分析相结合，使我们一方面能够鉴定出无法通过直接免疫荧光进行区分的抗原相似菌株（菌株1），另一方面能够在对新物种菌株进行DNA - ADN杂交之前采用更精确的鉴定方法（菌株2）。
