Ward K H, Olson M E, Lam K, Costerton J W
Department of Biological Sciences, University of Calgary, Alberta, Canada.
J Med Microbiol. 1992 Jun;36(6):406-13. doi: 10.1099/00222615-36-6-406.
The ability of rabbits to clear an intraperitoneal injection of Pseudomonas aeruginosa in the presence or absence of a surgically implanted peritoneal device was investigated. Sham-operated rabbits without an implant eliminated a P. aeruginosa challenge of 5 x 10(6) cfu/ml; lavage fluid and peritoneal tissues became culture-negative within 96 h. However, peritonitis developed in rabbits that were given the same number of bacteria in the presence of an implant; high bacterial counts were recovered from the lavage fluid and the device itself. Scanning and transmission electronmicroscopy revealed bacterial biofilms on the surface of the device. Insertion of pre-colonised devices demonstrated a rapid multiplication of sessile organisms within the resulting bacterial biofilm. Counts reached a plateau of about 1 x 10(7) cfu/cm2 of Silastic by day 16 and fluctuated around this level until the end of the study. Pre-immunisation with formalin-killed whole cells of P. aeruginosa did not reduce this bacterial growth despite high levels of specific IgG. The results confirm the failure of peritoneal defences to clear an infection in the presence of an implant following either challenge with planktonic bacteria or insertion of a pre-colonised device, and demonstrate the rapid development of bacterial biofilms on the surface of the implant which appear to protect the bacteria from host defences, even when primed by pre-immunisation.
研究了在存在或不存在手术植入的腹膜装置的情况下，兔子清除腹腔注射铜绿假单胞菌的能力。未植入装置的假手术兔子能够清除5×10(6) cfu/ml的铜绿假单胞菌攻击；灌洗液和腹膜组织在96小时内培养转阴。然而，在植入装置的情况下给予相同数量细菌的兔子发生了腹膜炎；从灌洗液和装置本身中回收了高细菌计数。扫描和透射电子显微镜显示装置表面有细菌生物膜。插入预先定植的装置表明，在形成的细菌生物膜内固着生物迅速繁殖。到第16天，硅橡胶表面的细菌计数达到约1×10(7) cfu/cm2的平台期，并在该水平附近波动直至研究结束。尽管有高水平的特异性IgG，但用甲醛灭活的铜绿假单胞菌全细胞进行预免疫并不能减少这种细菌生长。结果证实，在受到浮游细菌攻击或插入预先定植的装置后，腹膜防御在存在植入物的情况下无法清除感染，并证明植入物表面细菌生物膜迅速形成，这似乎保护细菌免受宿主防御，即使进行了预免疫。
