Kennedy M J, Johnson A M, Volz P A, Neely A N, Yancey R J
Therapeutics Research, Upjohn Company, Kalamazoo, MI 49001.
J Med Microbiol. 1992 Jun;36(6):428-36. doi: 10.1099/00222615-36-6-428.
The virulence and adhesive properties of 50 isolates of Candida albicans serotypes A and B collected over 6 years from 48 paediatric burn patients were examined to provide more detailed information about candidal pathogenesis in burn patients and to examine the relevance of the commonly used epithelial cell adhesion assay for determining fungal virulence. The isolates represented a fair distribution of serotypes (29 isolates were serotype A and 21 isolates were serotype B) and a total of 28 serotype-biotype combinations were found; 32% of the serotype-biotype combinations appeared only once, while 44% of the isolates showed similar biotype tests for two of three digits. Adhesion of the isolates to plastic and to buccal epithelial cells (BECs) was examined and compared after growth in a chemically defined medium. There were significant differences in the adhesion of individual isolates to plastic or BECs, but no correlation was found between biotype and adhesiveness. Serotype B isolates were found to be more adhesive to BECs (p less than 0.05) but not to plastic. There was no apparent correlation between candidal adhesiveness and site of isolation from these patients (autografts, blood, faeces, throat swabs, tracheal aspirates, wounds and intravenous catheters), although isolates from catheters were generally less adhesive to epithelial cells. Virulence in a systemic infection mouse model revealed that there were significant differences in virulence between isolates, but no correlation was found between virulence and the biotype, serotype or site of isolation. Similarly, no correlation was found between virulence and adhesiveness or cell-surface hydrophobicity.(ABSTRACT TRUNCATED AT 250 WORDS)
对6年间从48名小儿烧伤患者中分离出的50株A和B血清型白色念珠菌的毒力和黏附特性进行了检测，以提供有关烧伤患者念珠菌发病机制的更详细信息，并检验常用的上皮细胞黏附试验在确定真菌毒力方面的相关性。这些分离株代表了血清型的合理分布（29株为A血清型，21株为B血清型），共发现28种血清型-生物型组合；32%的血清型-生物型组合仅出现一次，而44%的分离株在三位数字中的两位显示出相似的生物型检测结果。在化学成分确定的培养基中生长后，检测并比较了分离株对塑料和颊上皮细胞（BEC）的黏附情况。各分离株对塑料或BEC的黏附存在显著差异，但生物型与黏附性之间未发现相关性。发现B血清型分离株对BEC的黏附性更强（p小于0.05），但对塑料的黏附性不强。念珠菌的黏附性与从这些患者身上分离的部位（自体移植组织、血液、粪便、咽拭子、气管吸出物、伤口和静脉导管）之间没有明显相关性，尽管从导管分离出的菌株通常对上皮细胞的黏附性较弱。在全身性感染小鼠模型中的毒力显示，分离株之间的毒力存在显著差异，但毒力与生物型、血清型或分离部位之间未发现相关性。同样，毒力与黏附性或细胞表面疏水性之间也未发现相关性。（摘要截短至250字）
