Al-Hashimi Hashim M
Department of Chemistry and Biophysics Research Division, University of Michigan, Ann Arbor, MI 48109, USA.
Chembiochem. 2005 Sep;6(9):1506-19. doi: 10.1002/cbic.200500002.
The ever-increasing cellular roles ascribed to RNA raise fundamental questions regarding how a biopolymer composed of only four chemically similar building-block nucleotides achieves such functional diversity. Here, I discuss how RNA achieves added mechanistic and chemical complexity by undergoing highly controlled conformational changes in response to a variety of cellular signals. I examine pathways for achieving selectivity in these conformational changes that rely to different extents on the structure and dynamics of RNA. Finally, I review solution-state NMR techniques that can be used to characterize RNA structural dynamics and its relationship to function.
RNA在细胞中的作用不断增加，这引发了一些基本问题，即由仅四种化学性质相似的构建核苷酸组成的生物聚合物如何实现如此多样的功能。在这里，我将讨论RNA如何通过响应各种细胞信号进行高度可控的构象变化来实现额外的机制和化学复杂性。我研究了在这些构象变化中实现选择性的途径，这些途径在不同程度上依赖于RNA的结构和动力学。最后，我回顾了可用于表征RNA结构动力学及其与功能关系的溶液态核磁共振技术。
