Laczka-Osyczka A, Laczka M, Kasugai S, Ohya K
Department of Animal Biochemistry, Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
J Biomed Mater Res. 1998 Dec 5;42(3):433-42. doi: 10.1002/(sici)1097-4636(19981205)42:3<433::aid-jbm13>3.0.co;2-h.
Previous studies have shown different macrophage responses to three compact pellets (with slightly different chemical composition) of gel-derived bioactive glass-ceramics of the CaO-P2O5-SiO2 system. In the present study primary bone marrow cells directed in vitro to form osteoblastic or osteoclastic cells were cultivated on glass slides coated by these three glass-ceramics. Glass slides were used as controls. In osteoblastic cultures alkaline phosphatase activity varied, depending on the type of coatings. Northern analysis showed high mRNA expressions of bone-related proteins on both the glass-ceramics and control glass. Mineralized nodules were not formed on the control glass, but coating glass slides with the glass-ceramics promoted mineralization without any substantial differences between the types of coatings. In osteoclastic cultures, the normal morphology of tartrate resistant acid phosphatase-positive multinucleated cells on standard culture plates was altered on the control glass and the glass-ceramics. The number of these cells differed, depending on the type of coatings, with no particular differences in the arrangement of F-actin by these cells. These analyses demonstrate complete biocompatibility of the glass-ceramic coatings but not the control glass, on which the cells failed to form mineralized nodules. The phenotype expression of the cells appeared to be influenced by microstructure, surface roughness, and the general character of the coatings rather than their surface reactivity.
先前的研究表明，巨噬细胞对CaO-P2O5-SiO2系统凝胶衍生生物活性玻璃陶瓷的三种致密颗粒（化学成分略有不同）有不同反应。在本研究中，将体外定向形成成骨细胞或破骨细胞的原代骨髓细胞培养在涂有这三种玻璃陶瓷的载玻片上。以载玻片作为对照。在成骨细胞培养中，碱性磷酸酶活性因涂层类型而异。Northern分析显示，玻璃陶瓷和对照玻璃上与骨相关蛋白的mRNA表达均很高。对照玻璃上未形成矿化结节，但用玻璃陶瓷涂覆载玻片可促进矿化，且不同类型涂层之间无实质性差异。在破骨细胞培养中，标准培养板上耐酒石酸酸性磷酸酶阳性多核细胞的正常形态在对照玻璃和玻璃陶瓷上均发生改变。这些细胞的数量因涂层类型而异，这些细胞的F-肌动蛋白排列没有特别差异。这些分析表明，玻璃陶瓷涂层具有完全的生物相容性，而对照玻璃则不具有，细胞在对照玻璃上无法形成矿化结节。细胞的表型表达似乎受微观结构、表面粗糙度和涂层的一般特性影响，而非其表面反应性。
