Metzger D, Losson R, Bornert J M, Lemoine Y, Chambon P
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Strasburg, France.
Nucleic Acids Res. 1992 Jun 11;20(11):2813-7. doi: 10.1093/nar/20.11.2813.
We have previously demonstrated that the human oestrogen receptor (hER) contains two transcriptional activation functions located in the N-terminal region (TAF-1) and in the hormone binding domain (TAF-2), which can act both independently and synergistically in a promoter- and cell-specific manner in animal cells. We have also demonstrated that hER can activate transcription from chimaeric oestrogen-responsive GAL1 promoters in yeast, and shown that transcriptional activation was due to TAF-1, whereas TAF-2 showed little, if any, transcriptional activity on these promoters. By using a more complex promoter derived from the URA3 gene, we now show that TAF-2 is also active in yeast, and that the activities of TAF-1 and TAF-2 are promoter-context-specific in yeast. We also confirm that the agonistic activity of 4-hydroxytamoxifen (OHT) can be ascribed to the activity of TAF-1.
我们之前已经证明，人类雌激素受体（hER）包含两个转录激活功能域，分别位于N端区域（TAF-1）和激素结合域（TAF-2），它们在动物细胞中能够以启动子和细胞特异性的方式独立或协同发挥作用。我们还证明，hER可以激活酵母中嵌合雌激素反应性GAL1启动子的转录，并表明转录激活是由TAF-1引起的，而TAF-2在这些启动子上几乎没有转录活性。通过使用源自URA3基因的更复杂的启动子，我们现在表明TAF-2在酵母中也具有活性，并且TAF-1和TAF-2的活性在酵母中是启动子背景特异性的。我们还证实，4-羟基他莫昔芬（OHT）的激动活性可归因于TAF-1的活性。
