Robinson Anandakathir, Fang Jim-Min, Chou Pi-Tai, Liao Kuang-Wen, Chu Rea-Min, Lee Shyh-Jye
Department of Chemistry, National Taiwan University, Taipei, 106, Taiwan.
Chembiochem. 2005 Oct;6(10):1899-905. doi: 10.1002/cbic.200500112.
We report the encapsulation of quantum dots with biologically important beta-N-acetylglucosamine (GlcNAc) in different ratios, together with studies of their specific/sensitive multivalent interactions with lectins and sperm by fluorimetry, transmission electron microscopy, dynamic light scattering microscopy, confocal imaging techniques, and flow cytometry. These GlcNAc-encapsulated quantum dots (QDGLNs) specifically bind to wheat germ agglutinin, and cause fluorescence quenching and aggregation. Further studies of QDGLNs and the mannose-encapsulated QDs (QDMANs) with sperm revealed site-specific interactions, in which QDGLNs bind to the head of the sperm, while QDMANs spread over the whole sperm body.
我们报告了以不同比例用具有生物学重要性的β-N-乙酰葡糖胺（GlcNAc）包裹量子点的情况，以及通过荧光测定法、透射电子显微镜、动态光散射显微镜、共聚焦成像技术和流式细胞术对其与凝集素和精子的特异性/敏感多价相互作用的研究。这些GlcNAc包裹的量子点（QDGLNs）特异性结合小麦胚芽凝集素，并导致荧光猝灭和聚集。对QDGLNs和用甘露糖包裹的量子点（QDMANs）与精子的进一步研究揭示了位点特异性相互作用，其中QDGLNs结合到精子头部，而QDMANs分布在整个精子体上。
