Xie Hai-Yan, Xie Min, Zhang Zhi-Ling, Long Yan-Min, Liu Xin, Tang Ming-Liang, Pang Dai-Wen, Tan Zheng, Dickinson Calum, Zhou Wuzong
School of Life Science and Technology, Beijing Institute of Technology, Beijing, PR China.
Bioconjug Chem. 2007 Nov-Dec;18(6):1749-55. doi: 10.1021/bc060387g. Epub 2007 Sep 26.
A simple and convenient strategy has been put forward to fabricate smart fluorescent magnetic wheat germ agglutinin-modified trifunctional nanospheres (WGA-TFNS) for recognition of human prostate carcinoma DU-145 cells which are surface-expressed with sialic acid and N-acetylglucosamine. These TFNS can be easily manipulated, tracked, and conveniently used to capture and separate target cells. The presence of wheat germ agglutinin on the surface of WGA-TFNS was confirmed by FTIR, biorecognition of carboxymethyl chitin-modified quantum dots (CM-CT-QDs), and bacterium Staphylococcus aureus. The success in recognizing DU-145 cells by the WGA-TFNS indicates that WGA-TFNS could be applicable.
已经提出了一种简单便捷的策略来制备智能荧光磁性麦胚凝集素修饰的三功能纳米球（WGA-TFNS），用于识别表面表达唾液酸和N-乙酰葡糖胺的人前列腺癌DU-145细胞。这些TFNS易于操作、追踪，并且可方便地用于捕获和分离靶细胞。通过傅里叶变换红外光谱（FTIR）、羧甲基几丁质修饰的量子点（CM-CT-QDs）的生物识别以及金黄色葡萄球菌，证实了WGA-TFNS表面存在麦胚凝集素。WGA-TFNS成功识别DU-145细胞表明其具有适用性。
