Brenner M, Braun C, Oster M, Gulko P S
The Robert S Boas Center for Genomics and Human Genetics, North Shore-LIJ Research Institute, 350 Community Drive, Manhasset, NY 11030, USA.
Ann Rheum Dis. 2006 Mar;65(3):306-11. doi: 10.1136/ard.2004.035246. Epub 2005 Sep 8.
To examine the potential usefulness of a novel thermal imaging technique to evaluate and monitor inflammatory arthritis activity in small joints using rat models, and to determine whether thermal changes can be used to detect preclinical stages of synovitis.
Three different rat strains were studied in a model of inflammatory arthritis of the ankle induced by an intra-articular (IA) injection of complete Freund's adjuvant (CFA), compared with the contralateral ankle injected with normal saline. Arthritis activity and severity scores, ankle diameters, pain related posture scores, and thermal images were obtained at 10 different times between 0 h (before induction) and day 7. The pristane induced arthritis (PIA) model was used to study preclinical synovitis. Thermal images were obtained at each time point using the TSA ImagIR system and were digitally analysed.
Rats developed similar ankle arthritis detected six hours after the IA injection of CFA, which persisted for seven days. All ankle clinical indices, including arthritis activity and severity scores, correlated significantly with ankle thermal imaging changes in the monoarthritis model (p<0.003). No thermal imaging changes were detected in preclinical stages of PIA. However, PIA onset coincided with increased ankle thermal signature.
Thermal measurements correlated significantly with arthritis activity and severity indices. The technique was highly sensitive and could measure directly two cardinal signs of inflammation (warmth and oedema, based on ankle diameter) in an area (ankle) that is less than half the size of a human interphalangeal joint, suggesting a potential use in drug trials or clinical practice.
使用大鼠模型研究一种新型热成像技术在评估和监测小关节炎症性关节炎活动方面的潜在用途，并确定热变化是否可用于检测滑膜炎的临床前期阶段。
在关节内（IA）注射完全弗氏佐剂（CFA）诱导的踝关节炎症性关节炎模型中研究三种不同的大鼠品系，并与注射生理盐水的对侧踝关节进行比较。在0小时（诱导前）至第7天之间的10个不同时间点获取关节炎活动和严重程度评分、踝关节直径、疼痛相关姿势评分和热图像。使用 pristane 诱导性关节炎（PIA）模型研究临床前期滑膜炎。使用 TSA ImagIR 系统在每个时间点获取热图像并进行数字分析。
在IA注射CFA后6小时检测到大鼠出现类似的踝关节关节炎，持续7天。在单关节炎模型中，所有踝关节临床指标，包括关节炎活动和严重程度评分，均与踝关节热成像变化显著相关（p<0.003）。在PIA的临床前期阶段未检测到热成像变化。然而，PIA发作与踝关节热信号增加同时发生。
热测量与关节炎活动和严重程度指标显著相关。该技术高度敏感，可直接测量面积（踝关节）小于人类指间关节一半大小区域内炎症的两个主要体征（基于踝关节直径的发热和水肿），表明在药物试验或临床实践中有潜在用途。
