Phan T G, Okame M, Nguyen T A, Nishio O, Okitsu S, Ushijima H
Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Arch Virol. 2005 Feb;150(2):371-7. doi: 10.1007/s00705-004-0401-0. Epub 2004 Oct 11.
A total of 517 fecal specimens collected from infants and children with acute gastroenteritis in Karachi city, Pakistan during 1990-1994 were examined for the presence of sapovirus by RT-PCR and sequence analysis methods. Sapovirus was identified in 17 of 517 (3.2%) specimens. Sapovirus was further clustered into three distinct genogroups (I, II and IV) and these presented 70.6%, 23.5% and 5.9%, respectively. Our results clearly indicated that sapovirus could be classified into 7 GI and 4 GII genotypes. It was noteworthy to point out that sapovirus detected among Pakistani infants and children with acute gastroenteritis demonstrated the great genetic diversity and presented novel sapovirus genotypes.
1990年至1994年期间，从巴基斯坦卡拉奇市患有急性肠胃炎的婴幼儿中收集了517份粪便标本，采用逆转录聚合酶链反应（RT-PCR）和序列分析方法检测其中是否存在札幌病毒。在517份标本中的17份（3.2%）中鉴定出了札幌病毒。札幌病毒进一步分为三个不同的基因组（I、II和IV），分别占70.6%、23.5%和5.9%。我们的结果清楚地表明，札幌病毒可分为7种GI基因型和4种GII基因型。值得指出的是，在巴基斯坦患有急性肠胃炎的婴幼儿中检测到的札幌病毒具有很大的遗传多样性，并呈现出新的札幌病毒基因型。
