RasGAP相关的核糖核酸内切酶G3BP对胎儿生长和新生儿存活的调控
Control of fetal growth and neonatal survival by the RasGAP-associated endoribonuclease G3BP.
作者信息
Zekri Latifa, Chebli Karim, Tourrière Hélène, Nielsen Finn C, Hansen Thomas V O, Rami Abdelhaq, Tazi Jamal
机构信息
Institut de Génétique Moléculaire de Montpellier UMR 5535, IFR 122, Centre National de Recherche Scientifique, 1919 route de Mende, 34293 Montpellier, France.
出版信息
Mol Cell Biol. 2005 Oct;25(19):8703-16. doi: 10.1128/MCB.25.19.8703-8716.2005.
Abstract
The regulation of mRNA stability plays a major role in the control of gene expression during cell proliferation, differentiation, and development. Here, we show that inactivation of the RasGAP-associated endoribonuclease (G3BP)-encoding gene leads to embryonic lethality and growth retardation. G3BP-/- mice that survived to term exhibited increased apoptotic cell death in the central nervous system and neonatal lethality. Both in mouse embryonic fibroblasts and during development, the absence of G3BP altered the expression of essential growth factors, among which imprinted gene products and growth arrest-specific mRNAs were outstanding. The results demonstrate that G3BP is essential for proper embryonic growth and development by mediating the coordinate expression of multiple imprinted growth-regulatory transcripts.
摘要
mRNA稳定性的调控在细胞增殖、分化和发育过程中的基因表达控制中起着重要作用。在此，我们表明，编码RasGAP相关核糖核酸内切酶（G3BP）的基因失活会导致胚胎致死和生长迟缓。存活至足月的G3BP-/-小鼠在中枢神经系统中表现出凋亡细胞死亡增加以及新生期致死。在小鼠胚胎成纤维细胞和发育过程中，G3BP的缺失均改变了必需生长因子的表达，其中印记基因产物和生长停滞特异性mRNA尤为突出。结果表明，G3BP通过介导多种印记生长调节转录本的协调表达，对胚胎的正常生长和发育至关重要。
相似文献
1
Control of fetal growth and neonatal survival by the RasGAP-associated endoribonuclease G3BP.RasGAP相关的核糖核酸内切酶G3BP对胎儿生长和新生儿存活的调控
Mol Cell Biol. 2005 Oct;25(19):8703-16. doi: 10.1128/MCB.25.19.8703-8716.2005.
2
RasGAP-associated endoribonuclease G3Bp: selective RNA degradation and phosphorylation-dependent localization.RasGAP相关的核糖核酸内切酶G3Bp：选择性RNA降解和磷酸化依赖性定位。
Mol Cell Biol. 2001 Nov;21(22):7747-60. doi: 10.1128/MCB.21.22.7747-7760.2001.
3
Cse1l is essential for early embryonic growth and development.Cse1l对早期胚胎的生长和发育至关重要。
Mol Cell Biol. 2001 Oct;21(20):7020-4. doi: 10.1128/MCB.21.20.7020-7024.2001.
4
Loss of the serine/threonine kinase fused results in postnatal growth defects and lethality due to progressive hydrocephalus.丝氨酸/苏氨酸激酶fused的缺失会导致出生后生长缺陷，并因进行性脑积水而致死。
Mol Cell Biol. 2005 Aug;25(16):7054-68. doi: 10.1128/MCB.25.16.7054-7068.2005.
5
Expression of mouse Coiled-coil-DIX1 (Ccd1), a positive regulator of Wnt signaling, during embryonic development.小鼠卷曲螺旋结构域蛋白DIX1（Ccd1）作为Wnt信号通路的正向调节因子在胚胎发育过程中的表达。
Gene Expr Patterns. 2006 Mar;6(3):325-30. doi: 10.1016/j.modgep.2005.06.013. Epub 2005 Dec 27.
6
Conditional inactivation of the mouse Hus1 cell cycle checkpoint gene.小鼠Hus1细胞周期检查点基因的条件性失活
Genomics. 2005 Aug;86(2):212-24. doi: 10.1016/j.ygeno.2005.04.007.
7
Cajal-Retzius cells and subplate neurons differentially express vesicular glutamate transporters 1 and 2 during development of mouse cortex.在小鼠皮质发育过程中，卡哈尔-雷茨乌斯细胞和板下神经元差异性表达囊泡谷氨酸转运体1和2。
Eur J Neurosci. 2007 Aug;26(3):615-23. doi: 10.1111/j.1460-9568.2007.05703.x. Epub 2007 Jul 25.
8
Distinct structural features of caprin-1 mediate its interaction with G3BP-1 and its induction of phosphorylation of eukaryotic translation initiation factor 2alpha, entry to cytoplasmic stress granules, and selective interaction with a subset of mRNAs.Caprin-1的独特结构特征介导了它与G3BP-1的相互作用、对真核翻译起始因子2α磷酸化的诱导、进入细胞质应激颗粒以及与一部分mRNA的选择性相互作用。
Mol Cell Biol. 2007 Mar;27(6):2324-42. doi: 10.1128/MCB.02300-06. Epub 2007 Jan 8.
9
Loss of Borealin/DasraB leads to defective cell proliferation, p53 accumulation and early embryonic lethality.Borealin/DasraB的缺失会导致细胞增殖缺陷、p53积累和早期胚胎致死。
Mech Dev. 2008 May-Jun;125(5-6):441-50. doi: 10.1016/j.mod.2008.01.011. Epub 2008 Feb 5.
10
Expansion and differentiation of neural progenitors derived from the human adult enteric nervous system.源自人类成人肠道神经系统的神经祖细胞的扩增与分化。
Gastroenterology. 2009 Dec;137(6):2063-2073.e4. doi: 10.1053/j.gastro.2009.06.038. Epub 2009 Jun 21.
引用本文的文献
1
G3BP1 maintains endothelial barrier integrity through dual mechanisms: direct stabilization of junction protein mRNAs and suppression of the inflammatory MYD88-ARNO-ARF6 pathway.G3BP1通过双重机制维持内皮屏障的完整性：直接稳定连接蛋白mRNA以及抑制炎症性的MYD88-ARNO-ARF6信号通路。
Angiogenesis. 2025 Aug 25;28(4):46. doi: 10.1007/s10456-025-09993-5.
2
Co-profiling of in situ RNA-protein interactions and transcriptome in single cells and tissues.单细胞和组织中原位RNA-蛋白质相互作用与转录组的联合分析
Nat Methods. 2025 Aug 11. doi: 10.1038/s41592-025-02774-4.
3
SNORD88B promotes proliferation and invasion of trophoblast by regulating G3BP1 m6 A modification and alternative splicing.SNORD88B通过调节G3BP1的m6A修饰和可变剪接促进滋养层细胞的增殖和侵袭。
Cell Mol Life Sci. 2025 Jun 5;82(1):225. doi: 10.1007/s00018-025-05758-x.
4
Stress granules: Guardians of cellular health and triggers of disease.应激颗粒：细胞健康的守护者与疾病的触发因素
Neural Regen Res. 2026 Feb 1;21(2):588-597. doi: 10.4103/NRR.NRR-D-24-01196. Epub 2025 Feb 24.
5
Stress granule-related genes during embryogenesis of an invertebrate chordate.一种无脊椎脊索动物胚胎发育过程中与应激颗粒相关的基因。
Front Cell Dev Biol. 2024 Aug 1;12:1414759. doi: 10.3389/fcell.2024.1414759. eCollection 2024.
6
Role(s) of G3BPs in Human Pathogenesis.G3BPs 在人类发病机制中的作用。
J Pharmacol Exp Ther. 2023 Oct;387(1):100-110. doi: 10.1124/jpet.122.001538. Epub 2023 Jul 19.
7
Stress granules sequester Alzheimer's disease-associated gene transcripts and regulate disease-related neuronal proteostasis.应激颗粒隔离与阿尔茨海默病相关的基因转录本，并调节与疾病相关的神经元蛋白稳态。
Aging (Albany NY). 2023 May 22;15(10):3984-4011. doi: 10.18632/aging.204737.
8
Multiple functions of stress granules in viral infection at a glance.应激颗粒在病毒感染中的多种功能一览。
Front Microbiol. 2023 Mar 1;14:1138864. doi: 10.3389/fmicb.2023.1138864. eCollection 2023.
9
RNA degradation eliminates developmental transcripts during murine embryonic stem cell differentiation via CAPRIN1-XRN2.RNA 降解通过 CAPRIN1-XRN2 在小鼠胚胎干细胞分化过程中消除发育性转录本。
Dev Cell. 2022 Dec 19;57(24):2731-2744.e5. doi: 10.1016/j.devcel.2022.11.014. Epub 2022 Dec 9.
10
Tryptophan mutations in G3BP1 tune the stability of a cellular signaling hub by weakening transient interactions with Caprin1 and USP10.G3BP1 中的色氨酸突变通过削弱与 Caprin1 和 USP10 的瞬时相互作用来调节细胞信号枢纽的稳定性。
J Biol Chem. 2022 Dec;298(12):102552. doi: 10.1016/j.jbc.2022.102552. Epub 2022 Sep 29.
本文引用的文献
1
Rasputin, more promiscuous than ever: a review of G3BP.拉斯普京，比以往更加滥交：G3BP综述
Int J Dev Biol. 2004 Dec;48(10):1065-77. doi: 10.1387/ijdb.041893ki.
2
Vaccinia virus intermediate stage transcription is complemented by Ras-GTPase-activating protein SH3 domain-binding protein (G3BP) and cytoplasmic activation/proliferation-associated protein (p137) individually or as a heterodimer.痘苗病毒中间阶段转录可分别由Ras鸟苷三磷酸酶激活蛋白SH3结构域结合蛋白（G3BP）和细胞质激活/增殖相关蛋白（p137）单独或作为异二聚体进行互补。
J Biol Chem. 2004 Dec 10;279(50):52210-7. doi: 10.1074/jbc.M411033200. Epub 2004 Oct 6.
3
Bringing the role of mRNA decay in the control of gene expression into focus.聚焦mRNA衰变在基因表达调控中的作用。
Trends Genet. 2004 Oct;20(10):491-7. doi: 10.1016/j.tig.2004.07.011.
4
Regulation of cytoplasmic stress granules by apoptosis-inducing factor.凋亡诱导因子对细胞质应激颗粒的调控
J Cell Sci. 2004 Sep 1;117(Pt 19):4461-8. doi: 10.1242/jcs.01356. Epub 2004 Aug 17.
5
Sequential dimerization of human zipcode-binding protein IMP1 on RNA: a cooperative mechanism providing RNP stability.人zipcode结合蛋白IMP1在RNA上的顺序二聚化：一种提供核糖核蛋白稳定性的协同机制。
Nucleic Acids Res. 2004 Aug 16;32(14):4368-76. doi: 10.1093/nar/gkh754. Print 2004.
6
Epigenetic regulation of mammalian genomic imprinting.哺乳动物基因组印记的表观遗传调控。
Curr Opin Genet Dev. 2004 Apr;14(2):188-95. doi: 10.1016/j.gde.2004.01.005.
7
The insulin-like growth factor mRNA binding-protein IMP-1 and the Ras-regulatory protein G3BP associate with tau mRNA and HuD protein in differentiated P19 neuronal cells.胰岛素样生长因子mRNA结合蛋白IMP-1和Ras调节蛋白G3BP在分化的P19神经元细胞中与tau mRNA和HuD蛋白相关联。
J Neurochem. 2004 May;89(3):613-26. doi: 10.1111/j.1471-4159.2004.02371.x.
8
The enzymes and control of eukaryotic mRNA turnover.真核生物mRNA周转的酶及调控
Nat Struct Mol Biol. 2004 Feb;11(2):121-7. doi: 10.1038/nsmb724.
9
GeneChip analysis of hippocampal gene expression profiles of short- and long-attack-latency mice: technical and biological implications.短攻击潜伏期和长攻击潜伏期小鼠海马基因表达谱的基因芯片分析：技术和生物学意义
J Neurosci Res. 2003 Dec 1;74(5):701-16. doi: 10.1002/jnr.10800.
10
Deubiquitination, a new player in Golgi to endoplasmic reticulum retrograde transport.去泛素化，高尔基体到内质网逆行转运中的新角色。
J Biol Chem. 2003 Dec 26;278(52):51989-92. doi: 10.1074/jbc.C300451200. Epub 2003 Oct 30.
Zotero 插件