Frösch D, Westphal C, Bachhuber K
Ultramicroscopy. 1985;17(2):141-6. doi: 10.1016/0304-3991(85)90007-5.
Chemical and physical data of two electron microscopic embedding media (the non-polar epoxy resin Epon 812 and the polar melamine resin Nanoplast FB 101) suggest that less kinetic energy must be applied for cutting a section from a Nanoplast block than from an Epon block of the same hardness and that, consequently, the cutting qualities of Nanoplast are better. To test this hypothesis, normal and extremely thin sections of Epon- and Nanoplast-embedded horse spleen ferritin micropellets were reembedded and resectioned for a determination of thickness and surface roughness. The ease with which extremely thin sections can be cut from the Nanoplast resin (8 nm versus 15 nm in Epon) and the smooth surface of these sections support the hypothesis that the cutting quality of an embedding material is determined primarily by its energy balance, i.e. by the kinetic energy which must be introduced for sectioning and the bonding energy which is released exothermically from a polymer while being sectioned.
两种电子显微镜包埋介质（非极性环氧树脂Epon 812和极性三聚氰胺树脂Nanoplast FB 101）的化学和物理数据表明，从相同硬度的Nanoplast块中切取切片所需的动能比从Epon块中切取时要少，因此，Nanoplast的切片质量更好。为了验证这一假设，将用Epon和Nanoplast包埋的马脾铁蛋白微颗粒的常规超薄切片重新包埋并再次切片，以测定厚度和表面粗糙度。从Nanoplast树脂中能够轻松切出超薄切片（8纳米，而Epon中为15纳米）以及这些切片表面的光滑度支持了这样的假设，即包埋材料的切片质量主要由其能量平衡决定，也就是说，由切片时必须引入的动能以及聚合物在切片时放热释放的结合能决定。
