全血中硫胺素或硫胺素磷酸酯的测定值不取决于所使用的抗凝剂。
Assay values for thiamine or thiamine phosphate esters in whole blood do not depend on the anticoagulant used.
作者信息
Ihara Hiroshi, Matsumoto Takayuki, Shino Yoshio, Hashizume Naotaka
机构信息
Department of Laboratory Medicine, Ohashi Hospital, Toho University Medical Center, 2-17-6 Ohashi, Meguro, Tokyo, Japan.
出版信息
J Clin Lab Anal. 2005;19(5):205-8. doi: 10.1002/jcla.20079.
We compared the whole blood, plasma, and erythrocyte (red blood cell (RBC)) concentrations of thiamine and thiamine phosphate esters in the presence of heparin or EDTA as anticoagulants. Three blood specimens were collected from each of 24 healthy volunteers into evacuated collection tubes containing the following anticoagulants: heparin, Na2EDTA, or K2EDTA. The concentrations of nonphosphorylated free thiamine (T), thiamine monophosphate (TMP), thiamine diphosphate (TDP), and thiamine triphosphate (TTP) were determined by the NH2-column HPLC method. The anticoagulant used had no effect on the concentrations obtained in whole blood and plasma of thiamine or any of the above thiamine compounds (P>0.05). RBCs were isolated by centrifugation and washed with isotonic saline, and the cell counts of the washed cells were adjusted to their whole blood values. In the washed RBCs with any anticoagulant, the concentrations of T, TMP, and TDP expressed either as nmol/L of whole blood or a ratio to hemoglobin were significantly lower (P<0.05) than those in whole blood.
我们比较了在使用肝素或乙二胺四乙酸（EDTA）作为抗凝剂的情况下，全血、血浆和红细胞（红血球，RBC）中硫胺素及硫胺素磷酸酯的浓度。从24名健康志愿者中每人采集三份血样，分别放入含有以下抗凝剂的真空采血管中：肝素、乙二胺四乙酸二钠（Na2EDTA）或乙二胺四乙酸二钾（K2EDTA）。采用氨基柱高效液相色谱法测定非磷酸化游离硫胺素（T）、硫胺素单磷酸酯（TMP）、硫胺素二磷酸酯（TDP）和硫胺素三磷酸酯（TTP）的浓度。所用抗凝剂对全血和血浆中硫胺素或上述任何硫胺素化合物的浓度均无影响（P>0.05）。通过离心分离红细胞并用等渗盐水洗涤，将洗涤后细胞的细胞计数调整至全血值。在使用任何抗凝剂的洗涤红细胞中，以全血每升纳摩尔数或与血红蛋白的比值表示的T、TMP和TDP浓度均显著低于全血中的浓度（P<0.05）。
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