采用高效液相色谱法同时测定人血液和血清中的硫胺素及其磷酸酯。
Concomitant determination of thiamin and its phosphate esters in human blood and serum by high-performance liquid chromatography.
作者信息
Tallaksen C M, Bøhmer T, Bell H, Karlsen J
机构信息
Department of Medicine, Aker University Hospital, Oslo, Norway.
出版信息
J Chromatogr. 1991 Mar 8;564(1):127-36. doi: 10.1016/0378-4347(91)80075-n.
A high-performance liquid chromatographic method for the simultaneous determination of thiamin and thiamin phosphate esters in human blood or serum has been developed. The eluent consists of acetonitrile and phosphate buffer, in the ratios 90:10 (v/v) for the elution of thiamine and 60:40 (v/v) for the phosphate esters. The four compounds are eluted within 15 min. The detection limit is 13-16 fmol. Between-assay variation is 5-11%. Samples of whole blood and serum from 30 healthy adults were analysed. The following reference values were obtained for 15 females 15 males (nM, mean +/- S.D.). In serum: thiamin, 10.9 +/- 2.9/16.9 +/- 3.3; thiamin monophosphate, 8.3 +/- 1.5/3.7 +/- 1.5. In whole blood: thiamin, 29.6 +/- 10.0/33.4 +/- 10.4; thiamin monophosphate, 9.7 +/- 2.3/10.9 +/- 5.1; thiamin diphosphate, 121 +/- 29.6/165 +/- 40.4.
已开发出一种同时测定人血液或血清中硫胺素和硫胺素磷酸酯的高效液相色谱法。洗脱液由乙腈和磷酸盐缓冲液组成，洗脱硫胺素时二者比例为90:10（v/v），洗脱磷酸酯时为60:40（v/v）。这四种化合物在15分钟内洗脱完毕。检测限为13 - 16飞摩尔。批间变异为5 - 11%。对30名健康成年人的全血和血清样本进行了分析。获得了15名女性和15名男性的以下参考值（纳摩尔，平均值±标准差）。血清中：硫胺素，10.9±2.9/16.9±3.3；硫胺素单磷酸酯，8.3±1.5/3.7±1.5。全血中：硫胺素，29.6±10.0/33.4±10.4；硫胺素单磷酸酯，9.7±2.3/10.9±5.1；硫胺素二磷酸酯，121±29.6/165±40.4。
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