1999年至2003年从加拿大侵袭性脑膜炎球菌病病例中分离出的C群脑膜炎球菌的抗原和基因特征。

Antigenic and genetic characterization of serogroup C meningococci isolated from invasive meningococcal disease cases in Canada from 1999 to 2003.

作者信息

Law Dennis K S, Stoltz Jan, Henderson Averil M, Tsang Raymond S W

机构信息

Laboratory of Pathogenic Neisseria and Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Canada.

出版信息

Can J Microbiol. 2005 Jul;51(7):523-30. doi: 10.1139/w05-085.

DOI:10.1139/w05-085

PMID:16175200

Abstract

Four hundred and forty-two serogroup C Neisseria meningitidis isolates from individual invasive meningococcal disease (IMD) patients in Canada during the period 1999 to 2003 were analyzed. The majority (84%) of the serogroup C meningococci were characterized by the serotype antigen 2a and belonged to the clonal complex of electrophoretic type ET-15. However, after more than a decade of endemic disease as well as a number of outbreaks and many vaccination campaigns, both genetic and antigenic variants of the serogroup C serotype 2a meningococci were noted. Such variants include strains characterized as C:2a:P1.5 and C:2a:P1.7,1 as well as a non-serotypeable phenotype due to a mutational hot spot on the serotype 2a PorB outer-membrane protein. Meningococci characterized by the antigen formula B:2a:P1.5,2 and B:2a:P1.7,1 have also been found, which suggests capsule switching. Besides the clonal group of ET-15/ET-37, small numbers of serogroup C isolates were found to belong to the clonal complexes of ST-8 (Cluster A4), ST-41/44 (Lineage 3), ST-35, and ST-269.

摘要

对1999年至2003年期间从加拿大侵袭性脑膜炎球菌病（IMD）患者个体中分离出的442株C群脑膜炎奈瑟菌进行了分析。大多数（84%）C群脑膜炎球菌以血清型抗原2a为特征，属于电泳型ET-15的克隆复合体。然而，在经历了十多年的地方病流行以及多次疫情爆发和多次疫苗接种运动后，发现了C群血清型2a脑膜炎球菌的基因和抗原变体。这些变体包括被鉴定为C:2a:P1.5和C:2a:P1.7,1的菌株，以及由于血清型2a PorB外膜蛋白上的一个突变热点而导致的不可分型表型。还发现了以抗原公式B:2a:P1.5,2和B:2a:P1.7,1为特征的脑膜炎球菌，这表明存在荚膜转换。除了ET-15/ET-37克隆群外，还发现少数C群分离株属于ST-8（A4簇）、ST-41/44（谱系3）、ST-35和ST-269的克隆复合体。