Public Health Agency of Canada, Winnipeg, Manitoba;
Can J Infect Dis Med Microbiol. 2012 Fall;23(3):e55-9. doi: 10.1155/2012/131328.
Serogroup C meningococcal disease has been endemic in Canada since the early 1990s, with periods of hyperendemic disease documented in the past two decades. The present study characterized invasive serogroup C meningococci in Canada during the period from 2002 to 2009.
Serogroup C meningococci were serotyped using monoclonal antibodies. Their clonal types were identified by either multilocus enzyme electrophoresis or multilocus sequence typing.
The number of invasive serogroup C Neisseria meningitidis isolates received at the National Microbiology Laboratory (Winnipeg, Manitoba) for characterization has dropped from a high of 173 isolates in 2001 to just 17 in 2009, possibly related to the introduction of the serogroup C meningococcal conjugate vaccine. Before 2006, 80% to 95% of all invasive serogroup C meningococci belonged to the electrophoreic type (ET)-15 clonal type, and the ET-37 (but not ET-15) type only accounted for up to 5% of all isolates. However, beginning in 2006, the percentage of the ET-15 clonal type decreased while the ET-37 (but not ET-15) type increased from 27% in 2006 to 52% in 2009. The percentage of invasive serogroup C isolates not belonging to either ET-15 or ET-37 also increased. Most ET-15 isolates expressed the antigenic formula of C:2a:P1.7,1 or C:2a:P1.5. In contrast, the ET-37 (but not ET-15) isolates mostly expressed the antigens of C:2a:P1.5,2 or C:2a:P1.2.
A shift in the antigenic and clonal type of invasive serogroup C meningococi was noted. This finding suggests vigilance in the surveillance of meningoccocal disease is warranted.
自 20 世纪 90 年代初以来，C 群脑膜炎奈瑟菌在加拿大流行，在过去的二十年中已经有记录到高发和超高发的流行时期。本研究描述了 2002 年至 2009 年期间加拿大侵袭性 C 群脑膜炎奈瑟菌的特征。
使用单克隆抗体对 C 群脑膜炎奈瑟菌进行血清分型。通过多位点酶电泳或多位点序列分型确定其克隆型。
国家微生物学实验室（温尼伯，曼尼托巴省）收到的用于特征描述的侵袭性 C 群脑膜炎奈瑟菌分离株数量从 2001 年的 173 株高峰降至 2009 年的 17 株，这可能与 C 群脑膜炎球菌结合疫苗的引入有关。在 2006 年之前，所有侵袭性 C 群脑膜炎奈瑟菌的 80%至 95%属于电泳型（ET）-15 克隆型，而 ET-37（而非 ET-15）型仅占所有分离株的 5%。然而，从 2006 年开始，ET-15 克隆型的比例下降，而 ET-37（而非 ET-15）型的比例从 2006 年的 27%增加到 2009 年的 52%。不属于 ET-15 或 ET-37 的侵袭性 C 群分离株的比例也增加了。大多数 ET-15 分离株表达 C:2a:P1.7,1 或 C:2a:P1.5 的抗原表型。相比之下，ET-37（而非 ET-15）分离株主要表达 C:2a:P1.5,2 或 C:2a:P1.2 的抗原。
侵袭性 C 群脑膜炎奈瑟菌的抗原和克隆型发生了变化。这一发现表明有必要加强对脑膜炎奈瑟菌病的监测。
