通过分析5'非编码区对来自老挝人民民主共和国的经典猪瘟病毒进行基因分型。
Genetic typing of classical swine fever viruses from Lao PDR by analysis of the 5' non-coding region.
作者信息
Blacksell Stuart D, Khounsy Syseng, Boyle David B, Gleeson Laurence J, Westbury Harvey A, Mackenzie John S
机构信息
CSIRO Livestock Industries, Australian Animal Health Laboratory, Private Bag 24, 3220, Geelong, Victoria, Australia.
出版信息
Virus Genes. 2005 Dec;31(3):349-55. doi: 10.1007/s11262-005-3253-0.
The 5' non-coding region (5'-NCR) of 27 classical swine fever virus (CSFV) isolates from Lao People's Democratic Republic (Lao PDR) during 1997 and 1999 were amplified by RT-PCR. A 150-bp region of the 5'-NCR amplicons was analysed and compared with reference CSFV of European and Asian origin and a phylogenetic dendrogram constructed. Following analysis, all viruses were determined to belong to genogroup 2. Viruses from Lao PDR grouped on a geographical basis with the majority of northern/central isolates falling into subgroup 2.1 and southern/central isolates falling into subgroup 2.2. These results concur with previous studies of CSF viruses from Lao PDR, although this study recognized the first occurrence of subgroup 2.1 in southern Lao PDR.
1997年至1999年期间，采用逆转录聚合酶链反应（RT-PCR）扩增了来自老挝人民民主共和国（老挝）的27株经典猪瘟病毒（CSFV）分离株的5'非编码区（5'-NCR）。对5'-NCR扩增子的150个碱基对区域进行了分析，并与欧洲和亚洲来源的参考CSFV进行了比较，并构建了系统发育树状图。经过分析，所有病毒均被确定属于基因群2。来自老挝的病毒按地理位置分组，大多数北部/中部分离株属于2.1亚组，南部/中部分离株属于2.2亚组。这些结果与之前对老挝CSF病毒的研究结果一致，尽管本研究首次发现2.1亚组在老挝南部出现。
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