Kitaya Y, Azuma H, Kiyota M
Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Osaka, Japan.
Adv Space Res. 2005;35(9):1584-8. doi: 10.1016/j.asr.2005.03.039.
Microalgae culture is likely to play an important role in aquatic food production modules in bioregenerative systems for producing feeds for fish, converting CO2 to O2 and remedying water quality as well as aquatic higher plants. In the present study, the effects of culture conditions on the cellular multiplication of microalgae, Euglena gracilis, was investigated as a fundamental study to determine the optimum culture conditions for microalgae production in aquatic food production modules including both microalgae culture and fish culture systems. E. gracilis was cultured under conditions with five levels of temperatures (25-33 degrees C), three levels of CO2 concentrations (2-6%), five levels of O2 concentrations (10-30%), and six levels of photosynthetic photon flux (20-200 micromoles m-2 s-1). The number of Euglena cells in a certain volume of solution was monitored with a microscope under each environmental condition. The multiplication rate of the cells was highest at temperatures of 27-31 degrees C, CO2 concentration of 4%, O2 concentration of 20% and photosynthetic photon flux of about 100 micromoles m-2 s-1. The results demonstrate that E. gracilis could efficiently produce biomass and convert CO2 to O2 under relatively low light intensities in aquatic food production modules.
微藻培养在生物再生系统的水产食品生产模块中可能发挥重要作用，这些系统用于生产鱼类饲料、将二氧化碳转化为氧气、改善水质以及培养水生高等植物。在本研究中，作为一项基础研究，研究了培养条件对微藻纤细裸藻细胞增殖的影响，以确定在包括微藻培养和鱼类养殖系统的水产食品生产模块中微藻生产的最佳培养条件。纤细裸藻在五种温度水平（25 - 33摄氏度）、三种二氧化碳浓度水平（2 - 6%）、五种氧气浓度水平（10 - 30%）和六种光合光子通量水平（20 - 200微摩尔·米-2·秒-1）的条件下进行培养。在每种环境条件下，用显微镜监测一定体积溶液中的裸藻细胞数量。细胞增殖率在温度为27 - 31摄氏度、二氧化碳浓度为4%、氧气浓度为20%和光合光子通量约为100微摩尔·米-2·秒-1时最高。结果表明，在水产食品生产模块中，纤细裸藻在相对较低的光照强度下能够高效地生产生物质并将二氧化碳转化为氧气。
