Kutisova K, Kulda J, Cepicka I, Flegr J, Koudela B, Teras J, Tachezy J
Department of Parasitology, Faculty of Science, Charles University in Prague, Vinicná 7, Prague 2, 128 44, Czech Republic.
Parasitology. 2005 Sep;131(Pt 3):309-19. doi: 10.1017/s0031182005008000.
To clarify the taxonomy of trichomonads associated with human respiratory diseases, we examined a collection of axenic trichomonad strains isolated from the oral cavity and bronchi of patients from pulmonary diseases clinics in Tallin, Estonia. The oral and bronchial strains were compared mutually as well as with a reference strain of Trichomonas tenax, a common inhabitant of the human oral cavity, and other trichomonad species from humans and animals. Unexpectedly, the morphological studies, as well as DNA sequencing of ITS1-5.8S rRNA-ITS2 regions revealed that the Estonian strains belong to the genus Tetratrichomonas, with a high similarity to the avian species Tetratrichomonas gallinarum. None of the strains belonged to Trichomonas tenax. DNA fingerprinting using the RAPD method separated Estonian strains into 2 distinct groups: 'bronchial' consisting of 5 and 2 strains isolated from bronchi and 'oral' cavity, respectively, and oral consisting of 3 oral strains. Consistent differences between 'bronchial' and 'oral' groups were confirmed by analysis of ITS1-5.8S rRNA-ITS2 sequences. Our results have revealed novel trichomonad species of the human oral cavity and bronchi.
为了阐明与人类呼吸道疾病相关的毛滴虫的分类，我们研究了从爱沙尼亚塔林肺病诊所患者的口腔和支气管中分离出的一系列无菌毛滴虫菌株。将口腔和支气管菌株相互比较，并与人类口腔常见寄居菌口腔毛滴虫的参考菌株以及来自人类和动物的其他毛滴虫物种进行比较。出乎意料的是，形态学研究以及ITS1-5.8S rRNA-ITS2区域的DNA测序表明，爱沙尼亚菌株属于四毛滴虫属，与禽类物种鸡四毛滴虫高度相似。没有一个菌株属于口腔毛滴虫。使用RAPD方法进行的DNA指纹分析将爱沙尼亚菌株分为2个不同的组：“支气管组”，分别由从支气管和口腔分离出的5株和2株组成，以及“口腔组”，由3株口腔菌株组成。通过对ITS1-5.8S rRNA-ITS2序列的分析，证实了“支气管组”和“口腔组”之间存在一致的差异。我们的研究结果揭示了人类口腔和支气管中的新型毛滴虫物种。
