Binolfi Andrés, Biasoli Marisa S, Luque Alicia G, Tosello María E, Magaró Hortensia M
CEREMIC, Facultad de Ciencias, Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina.
Med Mycol. 2005 Aug;43(5):431-7. doi: 10.1080/13693780400020147.
Candida dubliniensis is a recently described yeast species, closely related to Candida albicans. This work represents the first general survey of the carriage of C. dubliniensis in the oral cavities of HIV-positive patients in Argentina. We studied 133 strains isolated from 162 HIV-positive patients, using the following identification tests: chlamydospore production on corn meal agar with Tween 80; colony color on CHROMagar Candida media; differential growth at 45 degrees C on potato dextrose agar; D-xylose assimilation; chlamydospore formation on sunflower seed agar (SSA); carbohydrate assimilation profiles using the API 20 C Aux commercial kit and PCR using primers that hybridize to the class IV intron of the ACT1 gene. Out of the 133 strains, 21 were identified as C. dubliniensis, representing approximately 13% of the 162 patients in this study. From these data, we conclude that although the PCR assay is the most reliable method, clamydospore formation on SSA is an easier and less expensive test for the screening of C. dubliniensis in the routine laboratory. Our results show that C. dubliniensis has a high prevalence among HIV-positive patients in Argentina.
都柏林念珠菌是一种最近被描述的酵母菌种，与白色念珠菌密切相关。这项工作是对阿根廷HIV阳性患者口腔中都柏林念珠菌携带情况的首次全面调查。我们研究了从162名HIV阳性患者中分离出的133株菌株，采用了以下鉴定试验：在含吐温80的玉米粉琼脂上产厚垣孢子；在CHROMagar念珠菌培养基上的菌落颜色；在马铃薯葡萄糖琼脂上45摄氏度的差异生长；D-木糖同化；在向日葵籽琼脂（SSA）上形成厚垣孢子；使用API 20 C Aux商业试剂盒的碳水化合物同化谱以及使用与ACT1基因IV类内含子杂交的引物进行PCR。在这133株菌株中，21株被鉴定为都柏林念珠菌，占本研究中162名患者的约13%。根据这些数据，我们得出结论，虽然PCR检测是最可靠的方法，但在常规实验室中，在SSA上形成厚垣孢子是一种更简便且成本更低的都柏林念珠菌筛查试验。我们的结果表明，都柏林念珠菌在阿根廷HIV阳性患者中具有较高的流行率。
