Wu Su-hui, Sun Jing-fen, Guo Shu-zhen
Department of Obstetrics and Gynecology, The First Hospital, Shanxi Medical University, Taiyuan 030001, China.
Chin J Integr Med. 2005 Sep;11(3):205-8. doi: 10.1007/BF02836506.
To compare the therapeutic effect of Compound Recipe Gengniankang ( GNK) with that of hormone replacement treatment (HRT) on climacteric female rats with osteoporosis, and to investigate the roles of estrogen and estrogen receptors in the mechanism of osteoporosis.
Climacteric female rats with osteoporosis were chosen and divided into three groups (GNK group, HRT group and control group). Apoptosis of ovarian granulose cells was measured by terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) assay. Serum level of estradiol (E(2)), follicle stimulating hormone (FSH), luteinizing hormone (LH) were determined by the method of radioimmunoassay (RIA). Reverse transcriptase polymerase chain reaction (RT-PCT) technology was used to evaluate the expression of estrogen receptor (ER) in bone. Bone mineral density (BMD) was measured by double energy X-ray absorption (DEXA).
In the climacteric rats, BMD, serum E(2), ER mRNA expression in bone decreased remarkably, and serum FSH, LH and apoptosis of ovarian granulose cells increased obviously. After treating with GNK, all the indexes were reversed except serum E(2). The increase of E(2) was not significant.
GNK is effective on climacteric osteoporosis female rats. Its role is performed not by increasing serum E(2) but by enhancing ER in the bone and inhibiting apoptosis of ovarian granulose cells. GNK can deter further exhaustion of ovarian function.
比较复方更年康（GNK）与激素替代疗法（HRT）对绝经后骨质疏松雌性大鼠的治疗效果，并探讨雌激素及雌激素受体在骨质疏松发病机制中的作用。
选取绝经后骨质疏松雌性大鼠，分为三组（GNK组、HRT组和对照组）。采用末端脱氧核苷酸转移酶介导的缺口末端标记法（TUNEL）检测卵巢颗粒细胞凋亡。采用放射免疫分析法（RIA）测定血清雌二醇（E₂）、卵泡刺激素（FSH）、黄体生成素（LH）水平。采用逆转录聚合酶链反应（RT-PCR）技术评估骨组织中雌激素受体（ER）的表达。采用双能X线吸收法（DEXA）测量骨密度（BMD）。
绝经后大鼠骨密度、血清E₂、骨组织ER mRNA表达显著降低，血清FSH、LH及卵巢颗粒细胞凋亡明显增加。给予GNK治疗后，除血清E₂外，其余指标均逆转。E₂升高不显著。
GNK对绝经后骨质疏松雌性大鼠有效。其作用机制不是通过升高血清E₂，而是通过增强骨组织中ER表达及抑制卵巢颗粒细胞凋亡来实现的。GNK可延缓卵巢功能的进一步衰退。
