健康男性肌肉注射和口服多剂量给药方案后的血浆硫胺素浓度。

Plasma thiamine concentrations after intramuscular and oral multiple dosage regimens in healthy men.

作者信息

Royer-Morrot M J, Zhiri A, Paille F, Royer R J

机构信息

Département de Pharmacologie Clinique, CHRU, France.

出版信息

Eur J Clin Pharmacol. 1992;42(2):219-22. doi: 10.1007/BF00278489.

DOI:10.1007/BF00278489

PMID:1618256

Abstract

A novel liquid chromatographic method for the determination of thiamine in plasma has been developed and has been used to study plasma thiamine concentrations after multiple dosage regimens for 11 days. The method involves purification, concentration and analytical separation of thiochrome on-line, using a switching column system. Ten healthy men were given 500 mg thiamine i.m. once a day (Group 1) and ten were given 250 mg p.o. every 12 h (Group 2). The times to reach steady state (7 and 5.6 days for Groups 1 and 2, respectively) were not different (P greater than 0.05). The mean elimination half-life was 1.8 days. The mean minimum steady-state concentration after the oral regimen (23 micrograms.l-1) was 78% of that after the intramuscular regime (29 micrograms.l-1).

摘要

已开发出一种用于测定血浆中硫胺素的新型液相色谱方法，并已用于研究11天多次给药方案后的血浆硫胺素浓度。该方法采用切换柱系统，在线对硫色素进行纯化、浓缩和分析分离。10名健康男性每天一次肌肉注射500mg硫胺素（第1组），另外10名每12小时口服250mg（第2组）。达到稳态的时间（第1组和第2组分别为7天和5.6天）无差异（P大于0.05）。平均消除半衰期为1.8天。口服给药方案后的平均最低稳态浓度（23μg·l-1）是肌肉注射给药方案后（29μg·l-1）的78%。