He H B, Deegan R J, Wood M, Wood A J
Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, TN 37232-6602.
J Chromatogr. 1992 Feb 14;574(2):213-8.
This paper describes the application of a window diagram technique to optimize the four components of eluent (sodium acetate, sodium heptanesulfonate, acetonitrile and pH adjusted by monochloroacetic acid), for complete separation of five catecholamine compounds and the internal standard (3,4-dihydroxybenzylamine, DHBA). In addition, studies were performed to address the problem of the variable recovery of DHBA from dog plasma due to a time-dependent loss of DHBA. We found that this phenomenon can be prevented by pH adjustment prior to addition of DHBA, allowing development of an accurate high-performance liquid chromatographic assay for plasma catecholamines in dogs.
本文描述了一种窗图技术的应用，用于优化洗脱液的四个组分（醋酸钠、庚烷磺酸钠、乙腈和用一氯乙酸调节的pH值），以实现五种儿茶酚胺化合物和内标物（3,4-二羟基苄胺，DHBA）的完全分离。此外，还进行了研究以解决由于DHBA随时间损失导致其从犬血浆中回收率变化的问题。我们发现，在添加DHBA之前通过调节pH值可以防止这种现象，从而开发出一种用于犬血浆儿茶酚胺的准确的高效液相色谱测定法。
