Odagiri Y, Karube T, Katayama H, Takemoto K
Department of Public Health, Saitama Medical School, Japan.
J Nutr. 1992 Jul;122(7):1553-8. doi: 10.1093/jn/122.7.1553.
The effect of a 30-d pretreatment with vitamin C (L-ascorbic acid) in the drinking water and vitamin E (all-rac-alpha-tocopherol) in the diet on the clastogenic activity induced by X-rays and 6-mercaptopurine was investigated in female ICR/Jcl mice by the bone-marrow micronucleus test. Prefeeding with vitamin E-deficient diets led to a significant decrease in serum vitamin E concentration and to an enhancement of micronucleus formation by X-rays in bone marrow cells. Although dietary supplementation with vitamin E significantly increased the vitamin E concentration in serum, it did not affect the frequency of X-ray-induced micronuclei. Treatment with a high level of vitamin C in drinking water was effective in protecting against micronucleus formation by X-rays. The increase in micronucleus frequency in the vitamin E-deficient mice compared with the mice fed vitamin E-normal diets was no longer observed when a high level of vitamin C in drinking water was given simultaneously. The most efficient protective action against X-rays was observed when vitamin E-supplemented diets and a high level of vitamin C in drinking water were used together as a pretreatment. Any combination of the vitamins did not affect the micronucleus induction by 6-mercaptopurine.
通过骨髓微核试验，在雌性ICR/Jcl小鼠中研究了饮用水中维生素C（L-抗坏血酸）和饮食中维生素E（消旋α-生育酚）进行30天预处理对X射线和6-巯基嘌呤诱导的致断裂活性的影响。用缺乏维生素E的饮食预喂养导致血清维生素E浓度显著降低，并增强了X射线诱导的骨髓细胞微核形成。尽管饮食中补充维生素E显著提高了血清中维生素E的浓度，但它并未影响X射线诱导的微核频率。饮用水中高水平的维生素C处理可有效预防X射线诱导的微核形成。当同时给予饮用水中高水平的维生素C时，与喂食正常维生素E饮食的小鼠相比，维生素E缺乏小鼠的微核频率增加不再出现。当将补充维生素E的饮食和饮用水中高水平的维生素C一起用作预处理时，观察到对X射线最有效的保护作用。维生素的任何组合均不影响6-巯基嘌呤诱导的微核形成。
