Shu Xiji, Liu Yaling, Ren Liang, He Fanggang, Zhou Hongyan, Liu Lijiang, Liu Liang
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2005;25(4):423-6. doi: 10.1007/BF02828213.
To determining the postmortem interval (PMI) through quantitative analysis of the DNA degradation of cell nucleus in human brain and spleen by using image analysis technique (IAT). The brain and spleen tissues from 32 cadavers with known PMI were collected, subjected to cell smear every 1 h within the first 5-36 h after death, stained by Feulgen-Van's staining, Three indices reflecting DNA in brain cells (astrocytes) and splenic lymphocytes, including integral optical density (IOD), average optical density (AOD), average gray (AG) were measured by employing the mage analysis instrument. The results showed that IOD and AOD declined and AG increased with the prolongation of dead time within 5-36 h. A correlation between the PMI and gray parameters (IOD, AOD and AG) was identified and the corresponding regression equation was obtained. The parameters (IOD, AOD and AG) were proved to be effective quantitative indicators for accurate estimation of PMI within 5-36 h after death.
运用图像分析技术（IAT），通过对人脑海马和脾脏细胞核DNA降解进行定量分析来确定死后间隔时间（PMI）。收集32例已知PMI的尸体的脑和脾脏组织，在死后最初5 - 36小时内每1小时制作细胞涂片，采用福尔根 - 范氏染色法染色，利用图像分析仪测量反映脑细胞（星形胶质细胞）和脾淋巴细胞中DNA的三个指标，包括积分光密度（IOD）、平均光密度（AOD）、平均灰度（AG）。结果显示，在5 - 36小时内，随着死亡时间的延长，IOD和AOD下降，AG升高。确定了PMI与灰度参数（IOD、AOD和AG）之间的相关性，并获得了相应的回归方程。证明这些参数（IOD、AOD和AG）是准确估计死后5 - 36小时内PMI的有效定量指标。
