Zhang Jin Xia, Huang Chen Yang, Ng Tzi Bun, Wang He Xiang
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China.
Appl Microbiol Biotechnol. 2006 Jul;71(3):304-9. doi: 10.1007/s00253-005-0139-y. Epub 2005 Oct 1.
Mating tests, internal transcribed spacer (ITS) sequence analysis, intergenic spacer 1-restriction fragment length polymorphism (IGS1-RFLP), IGS1 sequence analysis, and IGS2-RFLP analysis were carried out on isolates of 17 morphologically different Pleurotus mushrooms collected on Ferula sinkiangensis. The isolates were divided, based on mating tests and ITS sequence analysis, into two groups identical to P. eryngii var. ferulae and P. nebrodensis, respectively. Single spores from these two groups were incompatible, but those from P. eryngii var. ferulae and P. eryngii were compatible and combined to produce 56.25% dikaryon mycelia with clamp connections. The ITS of P. eryngii var. ferulae and P. nebrodensis (GenBank accession no. AY311408) were both 638 bp in size but differed by 3% in sequence. P. eryngii var. ferulae and P. eryngii (GenBank accession no. AY368658) were identical in ITS size and sequence. P. nebrodensis was the dominant population of Pleurotus mushroom growing on F. sinkiangensis. It exhibited genetic diversity. The two species could also be distinguished by IGSI-RFLP, similar to identification by mating tests and ITS sequence analysis. Difference in IGS1-RFLP existed between P. eryngii var. ferulae and P. nebrodensis. The sequence difference reached 2.28%. Both IGS1 size and IGS1-RFLP were similar among the different samples of P. nebrodensis. The 17 isolates were separated into five types based on IGS2 size and IGS2-RFLP, with both interspecies and extraspecies differences. P. nebrodensis exhibited polymorphism and was divided into four types. These results agreed with macroscopic differences. IGS2 might be the effective domain of genetically polymorphic ribosomal DNA in P. nebrodensis mushrooms found in Xinjiang, China.
对从新疆阿魏菇上采集的17种形态各异的侧耳属蘑菇菌株进行了交配试验、内转录间隔区（ITS）序列分析、基因间隔区1-限制性片段长度多态性（IGS1-RFLP）、IGS1序列分析和IGS2-RFLP分析。基于交配试验和ITS序列分析，这些菌株分别被分为与阿魏侧耳和白灵侧耳相同的两组。这两组的单孢子不亲和，但阿魏侧耳和杏鲍菇的单孢子亲和，并结合产生了56.25%具锁状联合的双核菌丝体。阿魏侧耳和白灵侧耳的ITS（GenBank登录号AY311408）大小均为638 bp，但序列差异为3%。阿魏侧耳和杏鲍菇（GenBank登录号AY368658）的ITS大小和序列相同。白灵侧耳是生长在新疆阿魏菇上的侧耳属蘑菇的优势种群，表现出遗传多样性。这两个物种也可以通过IGS1-RFLP区分，类似于通过交配试验和ITS序列分析进行的鉴定。阿魏侧耳和白灵侧耳之间存在IGS1-RFLP差异，序列差异达2.28%。白灵侧耳的不同样本间IGS1大小和IGS1-RFLP均相似。基于IGS2大小和IGS2-RFLP，这17个菌株被分为五种类型，存在种间和种外差异。白灵侧耳表现出多态性，被分为四种类型。这些结果与宏观差异一致。IGS2可能是中国新疆发现的白灵侧耳蘑菇中核糖体DNA遗传多态性的有效区域。
