Avin Farhat Ahmadi, Bhassu Subha, Tan Yee Shin, Shahbazi Pedram, Vikineswary Sabaratnam
Mushroom Research Centre (MRC), University of Malaya, 50603 Kuala Lumpur, Malaysia ; Division of Biotechnology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Mushroom Research Centre (MRC), University of Malaya, 50603 Kuala Lumpur, Malaysia ; Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
ScientificWorldJournal. 2014 Jan 21;2014:793414. doi: 10.1155/2014/793414. eCollection 2014.
Identification of edible mushrooms particularly Pleurotus genus has been restricted due to various obstacles. The present study attempted to use the combination of two variable regions of IGS1 and ITS for classifying the economically cultivated Pleurotus species. Integration of the two regions proved a high ability that not only could clearly distinguish the species but also served sufficient intraspecies variation. Phylogenetic tree (IGS1+ITS) showed seven distinct clades, each clade belonging to a separate species group. Moreover, the species differentiation was tested by AMOVA and the results were reconfirmed by presenting appropriate amounts of divergence (91.82% among and 8.18% within the species). In spite of achieving a proper classification of species by combination of IGS1 and ITS sequences, the phylogenetic tree showed the misclassification of the species of P. nebrodensis and P. eryngii var. ferulae with other strains of P. eryngii. However, the constructed median joining (MJ) network could not only differentiate between these species but also offer a profound perception of the species' evolutionary process. Eventually, due to the sufficient variation among and within species, distinct sequences, simple amplification, and location between ideal conserved ribosomal genes, the integration of IGS1 and ITS sequences is recommended as a desirable DNA barcode.
由于各种障碍，可食用蘑菇尤其是侧耳属蘑菇的鉴定受到了限制。本研究试图利用IGS1和ITS两个可变区的组合对经济栽培的侧耳属物种进行分类。这两个区域的整合显示出很高的能力，不仅可以清楚地区分物种，而且具有足够的种内变异。系统发育树（IGS1+ITS）显示出七个不同的分支，每个分支属于一个单独的物种组。此外，通过分子方差分析（AMOVA）对物种分化进行了测试，通过呈现适当的差异量（种间91.82%，种内8.18%）再次证实了结果。尽管通过IGS1和ITS序列的组合实现了对物种的正确分类，但系统发育树显示，纳博讷侧耳和阿魏侧耳与其他杏鲍菇菌株的物种分类有误。然而，构建的中介连接（MJ）网络不仅可以区分这些物种，还能对物种的进化过程提供深刻的认识。最终，由于种间和种内存在足够的变异、独特的序列、简单的扩增以及位于理想的保守核糖体基因之间的位置，建议将IGS1和ITS序列的整合作为一种理想的DNA条形码。
