He Xiao-Lan, Wu Bo, Li Qian, Peng Wei-Hong, Huang Zhong-Qian, Gan Bing-Cheng
Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China.
Biogas Institute of Ministry of Agriculture, 4-13 Renmin Rd. South, Chengdu, 610041, China.
Mol Biol Rep. 2016 Jun;43(6):573-82. doi: 10.1007/s11033-016-3982-2. Epub 2016 Apr 13.
The aims of this study are to assess the utility of the internal transcribed spacer (ITS) region, and partial translation elongation factor (EF1α) and RNA polymerase II (RPB2) genes, for differentiation of Bailinggu, P. eryngii, and P. nebrodensis; to reconstruct phylogenetic relationships between the three species; and to confirm the taxonomic status of Bailinggu based on ribosomal and protein-coding genes. Pairwise genetic distances between Bailinggu, P. eryngii, and related Pleurotus strains were calculated by using the p-distance model, and molecular phylogeny of these isolates was estimated based on ITS, RPB2, and EF1α using maximum parsimony and Bayesian methods. Differences in ITS, RPB2, and EF1α sequences show that Bailinggu, P. eryngii, and P. nebrodensis are distinct at the species level. Phylogenetic analyses reveal that P. eryngii is closer to P. nebrodensis than to Bailinggu. Sequence analyses of ribosomal and protein-coding genes confirm that P. eryngii var. tuoliensis is identical to Bailinggu. P. eryngii var. tuoliensis should be raised to species level or a new name should be introduced for Bailinggu after a thorough investigation into Pleurotus isolates from Ferula in Xinjiang Province. This study helps to resolve uncertainty regarding Bailinggu, P. eryngii and P. nebrodensis, improving the resource management of these strains. ITS, EF1α, and RPB2 sequences can be used to distinguish Bailinggu, P. eryngii and P. nebrodensis as three different species, and P. eryngii var. tuoliensis should be the scientific name for Bailinggu at present.
本研究的目的是评估内转录间隔区（ITS）区域、部分翻译延伸因子（EF1α）和RNA聚合酶II（RPB2）基因在区分白灵菇、杏鲍菇和阿魏侧耳中的作用；重建这三个物种之间的系统发育关系；并基于核糖体和蛋白质编码基因确定白灵菇的分类地位。利用p距离模型计算白灵菇、杏鲍菇及相关侧耳菌株之间的成对遗传距离，并基于ITS、RPB2和EF1α，采用最大简约法和贝叶斯方法估计这些分离株的分子系统发育。ITS、RPB2和EF1α序列的差异表明，白灵菇、杏鲍菇和阿魏侧耳在物种水平上是不同的。系统发育分析表明，杏鲍菇与阿魏侧耳的亲缘关系比与白灵菇更近。核糖体和蛋白质编码基因的序列分析证实，杏鲍菇的托里变种与白灵菇相同。在对新疆阿魏侧耳属分离株进行全面调查后，杏鲍菇的托里变种应提升到物种水平，或者应为白灵菇引入一个新名称。本研究有助于解决关于白灵菇、杏鲍菇和阿魏侧耳的不确定性，改善这些菌株的资源管理。ITS、EF1α和RPB2序列可用于区分白灵菇、杏鲍菇和阿魏侧耳为三个不同的物种，目前杏鲍菇的托里变种应为白灵菇的学名。
