• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Alternative lactose catabolic pathway in Lactococcus lactis IL1403.乳酸乳球菌IL1403中的替代乳糖分解代谢途径。
Appl Environ Microbiol. 2005 Oct;71(10):6060-9. doi: 10.1128/AEM.71.10.6060-6069.2005.
2
Identification and functional characterisation of cellobiose and lactose transport systems in Lactococcus lactis IL1403.乳酸乳球菌IL1403中纤维二糖和乳糖转运系统的鉴定及功能表征
Arch Microbiol. 2008 Mar;189(3):187-96. doi: 10.1007/s00203-007-0308-8. Epub 2007 Oct 2.
3
Genetic characterization of the CcpA-dependent, cellobiose-specific PTS system comprising CelB, PtcB and PtcA that transports lactose in Lactococcus lactis IL1403.对包含 CelB、PtcB 和 PtcA 的 CcpA 依赖性、纤维二糖特异性 PTS 系统进行遗传特征分析，该系统可在 Lactococcus lactis IL1403 中运输乳糖。
Int J Food Microbiol. 2011 Jan 31;145(1):186-94. doi: 10.1016/j.ijfoodmicro.2010.12.011. Epub 2010 Dec 22.
4
Structure and expression of the Lactococcus lactis gene for phospho-beta-galactosidase (lacG) in Escherichia coli and L. lactis.乳酸乳球菌磷酸β-半乳糖苷酶（lacG）基因在大肠杆菌和乳酸乳球菌中的结构与表达
J Gen Microbiol. 1989 Jul;135(7):1833-46. doi: 10.1099/00221287-135-7-1833.
5
ClaR--a novel key regulator of cellobiose and lactose metabolism in Lactococcus lactis IL1403.ClaR——乳酸乳球菌IL1403中纤维二糖和乳糖代谢的新型关键调节因子。
Appl Microbiol Biotechnol. 2015 Jan;99(1):337-47. doi: 10.1007/s00253-014-6067-y. Epub 2014 Sep 20.
6
Integration and gene replacement in the Lactococcus lactis lac operon: induction of a cryptic phospho-beta-glucosidase in LacG-deficient strains.乳酸乳球菌乳糖操纵子中的整合与基因置换：LacG缺陷型菌株中隐性磷酸β-葡萄糖苷酶的诱导
J Bacteriol. 1993 Aug;175(16):5168-75. doi: 10.1128/jb.175.16.5168-5175.1993.
7
GlaR (YugA)-a novel RpiR-family transcription activator of the Leloir pathway of galactose utilization in Lactococcus lactis IL1403.乳球菌乳糖利用 Leloir 途径中新型 RpiR 家族转录激活因子 GlaR（YugA）的鉴定。
Microbiologyopen. 2019 May;8(5):e00714. doi: 10.1002/mbo3.714. Epub 2018 Aug 11.
8
Heterologous expression of glycoside hydrolase family 2 and 42 β-galactosidases of lactic acid bacteria in Lactococcus lactis.在乳球菌中异源表达细菌糖苷水解酶家族 2 和 42 的β-半乳糖苷酶。
Syst Appl Microbiol. 2010 Oct;33(6):300-7. doi: 10.1016/j.syapm.2010.07.002. Epub 2010 Sep 6.
9
In vitro DNA binding of purified CcpA protein from Lactococcus lactis IL1403.来自乳酸乳球菌IL1403的纯化CcpA蛋白的体外DNA结合
Acta Biochim Pol. 2007;54(1):71-8. Epub 2007 Mar 14.
10
Molecular cloning of lactose genes in dairy lactic streptococci: the phospho-beta-galactosidase and beta-galactosidase genes and their expression products.乳制品乳酸链球菌中乳糖基因的分子克隆：磷酸β-半乳糖苷酶和β-半乳糖苷酶基因及其表达产物
Biochimie. 1988 Apr;70(4):461-73. doi: 10.1016/0300-9084(88)90083-1.

引用本文的文献

1
Comparative Genomic Analysis and Antimicrobial Resistance Profile of Strains Isolated from Raw Sheep Milk.从生羊乳中分离菌株的比较基因组分析及抗菌药物耐药性概况
Vet Sci. 2025 Jul 23;12(8):685. doi: 10.3390/vetsci12080685.
2
Polar-Region Soils as Novel Reservoir of Lactic Acid Bacteria from the Genus .极地土壤是乳杆菌属乳酸菌的新型蕴藏库。
Int J Mol Sci. 2024 Aug 30;25(17):9444. doi: 10.3390/ijms25179444.
3
The Presence of Plasmids in IL594 Determines Changes in the Host Phenotype and Expression of Chromosomal Genes.质粒在 IL594 中的存在决定了宿主表型和染色体基因表达的变化。
Int J Mol Sci. 2023 Jan 2;24(1):793. doi: 10.3390/ijms24010793.
4
Cross-utilization of β-galactosides and cellobiose in .β-半乳糖苷和纤维二糖在 … 的交叉利用。
J Biol Chem. 2020 Jul 31;295(31):10766-10780. doi: 10.1074/jbc.RA120.014029. Epub 2020 Jun 3.
5
Presence of galactose in precultures induces lacS and leads to short lag phase in lactose-grown Lactococcus lactis cultures.预培养物中存在半乳糖会诱导 lacS 的表达，导致乳糖生长的乳酸乳球菌培养物的迟滞期缩短。
J Ind Microbiol Biotechnol. 2019 Jan;46(1):33-43. doi: 10.1007/s10295-018-2099-0. Epub 2018 Nov 9.
6
The extracellular loop of Man-PTS subunit IID is responsible for the sensitivity of Lactococcus garvieae to garvicins A, B and C.Man-PTS 亚基 IID 的细胞外环负责乳球菌对 garvicin A、B 和 C 的敏感性。
Sci Rep. 2018 Oct 25;8(1):15790. doi: 10.1038/s41598-018-34087-2.
7
Large plasmidome of dairy Lactococcus lactis subsp. lactis biovar diacetylactis FM03P encodes technological functions and appears highly unstable.乳脂乳球菌乳亚种二乙酰亚种 FM03P 的大型质体基因组编码技术功能，且表现出高度不稳定性。
BMC Genomics. 2018 Aug 17;19(1):620. doi: 10.1186/s12864-018-5005-2.
8
GlaR (YugA)-a novel RpiR-family transcription activator of the Leloir pathway of galactose utilization in Lactococcus lactis IL1403.乳球菌乳糖利用 Leloir 途径中新型 RpiR 家族转录激活因子 GlaR（YugA）的鉴定。
Microbiologyopen. 2019 May;8(5):e00714. doi: 10.1002/mbo3.714. Epub 2018 Aug 11.
9
Preferred Hexoses Influence Long-Term Memory in and Induction of Lactose Catabolism by Streptococcus mutans.偏好的六碳糖影响变异链球菌的长期记忆和乳糖分解代谢的诱导。
Appl Environ Microbiol. 2018 Jul 2;84(14). doi: 10.1128/AEM.00864-18. Print 2018 Jul 15.
10
Insights into the Geobacillus stearothermophilus species based on phylogenomic principles.基于系统基因组学原理对嗜热栖热放线菌的见解。
BMC Microbiol. 2017 Jun 26;17(1):140. doi: 10.1186/s12866-017-1047-x.

本文引用的文献

1
High-Frequency Transformation, by Electroporation, of Lactococcus lactis subsp. cremoris Grown with Glycine in Osmotically Stabilized Media.在渗透压稳定的培养基中用甘氨酸培养的乳球菌乳亚种经电穿孔高频转化。
Appl Environ Microbiol. 1989 Dec;55(12):3119-23. doi: 10.1128/aem.55.12.3119-3123.1989.
2
Characterization, expression, and mutation of the Lactococcus lactis galPMKTE genes, involved in galactose utilization via the Leloir pathway.参与通过勒洛伊尔途径利用半乳糖的乳酸乳球菌galPMKTE基因的表征、表达及突变分析
J Bacteriol. 2003 Feb;185(3):870-8. doi: 10.1128/JB.185.3.870-878.2003.
3
Gene regulation in Lactococcus lactis: the gap between predicted and characterized regulators.乳酸乳球菌中的基因调控：预测的调控因子与已鉴定的调控因子之间的差距。
Antonie Van Leeuwenhoek. 2002 Aug;82(1-4):93-112.
4
The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403.乳酸乳球菌乳酸亚种IL1403的全基因组序列。
Genome Res. 2001 May;11(5):731-53. doi: 10.1101/gr.gr-1697r.
5
Catabolite repression mediated by the CcpA protein in Bacillus subtilis: novel modes of regulation revealed by whole-genome analyses.枯草芽孢杆菌中由CcpA蛋白介导的分解代谢物阻遏：全基因组分析揭示的新调控模式
Mol Microbiol. 2001 Mar;39(5):1366-81. doi: 10.1111/j.1365-2958.2001.02328.x.
6
Evaluation and characterization of catabolite-responsive elements (cre) of Bacillus subtilis.枯草芽孢杆菌分解代谢物反应元件（cre）的评估与表征
Nucleic Acids Res. 2000 Mar 1;28(5):1206-10. doi: 10.1093/nar/28.5.1206.
7
Regulation of expression of the Lactococcus lactis histidine operon.乳酸乳球菌组氨酸操纵子表达的调控
J Bacteriol. 1999 Apr;181(7):2026-37. doi: 10.1128/JB.181.7.2026-2037.1999.
8
Transcriptional activation of the glycolytic las operon and catabolite repression of the gal operon in Lactococcus lactis are mediated by the catabolite control protein CcpA.乳酸乳球菌中糖酵解las操纵子的转录激活和gal操纵子的分解代谢物阻遏由分解代谢物控制蛋白CcpA介导。
Mol Microbiol. 1998 Nov;30(4):789-98. doi: 10.1046/j.1365-2958.1998.01111.x.
9
Transcriptional activation of the Bacillus subtilis ackA gene requires sequences upstream of the promoter.枯草芽孢杆菌ackA基因的转录激活需要启动子上游的序列。
J Bacteriol. 1998 Nov;180(22):5961-7. doi: 10.1128/JB.180.22.5961-5967.1998.
10
Transcriptional regulation and evolution of lactose genes in the galactose-lactose operon of Lactococcus lactis NCDO2054.乳酸乳球菌NCDO2054半乳糖-乳糖操纵子中乳糖基因的转录调控与进化
J Bacteriol. 1998 Sep;180(18):4893-902. doi: 10.1128/JB.180.18.4893-4902.1998.

乳酸乳球菌IL1403中的替代乳糖分解代谢途径。

Alternative lactose catabolic pathway in Lactococcus lactis IL1403.

作者信息

Aleksandrzak-Piekarczyk Tamara, Kok Jan, Renault Pierre, Bardowski Jacek

机构信息

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland.

出版信息

Appl Environ Microbiol. 2005 Oct;71(10):6060-9. doi: 10.1128/AEM.71.10.6060-6069.2005.

DOI:10.1128/AEM.71.10.6060-6069.2005
PMID:16204522
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1265982/
Abstract

In this study, we present a glimpse of the diversity of Lactococcus lactis subsp. lactis IL1403 beta-galactosidase phenotype-negative mutants isolated by negative selection on solid media containing cellobiose or lactose and X-Gal (5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside), and we identify several genes essential for lactose assimilation. Among these are ccpA (encoding catabolite control protein A), bglS (encoding phospho-beta-glucosidase), and several genes from the Leloir pathway gene cluster encoding proteins presumably essential for lactose metabolism. The functions of these genes were demonstrated by their disruption and testing of the growth of resultant mutants in lactose-containing media. By examining the ccpA and bglS mutants for phospho-beta-galactosidase activity, we showed that expression of bglS is not under strong control of CcpA. Moreover, this analysis revealed that although BglS is homologous to a putative phospho-beta-glucosidase, it also exhibits phospho-beta-galactosidase activity and is the major enzyme in L. lactis IL1403 involved in lactose hydrolysis.

摘要

在本研究中，我们展示了通过在含有纤维二糖或乳糖以及X-Gal（5-溴-4-氯-3-吲哚基-β-D-吡喃半乳糖苷）的固体培养基上进行负选择分离得到的乳酸乳球菌乳亚种IL1403β-半乳糖苷酶表型阴性突变体的多样性，并且我们鉴定了几个乳糖同化所必需的基因。其中包括ccpA（编码分解代谢物控制蛋白A）、bglS（编码磷酸-β-葡萄糖苷酶），以及来自Leloir途径基因簇的几个基因，这些基因编码的蛋白质可能是乳糖代谢所必需的。这些基因的功能通过对其进行破坏并检测所得突变体在含乳糖培养基中的生长情况得以证明。通过检测ccpA和bglS突变体的磷酸-β-半乳糖苷酶活性，我们发现bglS的表达不受CcpA的严格调控。此外，该分析表明，尽管BglS与一种假定的磷酸-β-葡萄糖苷酶同源，但它也表现出磷酸-β-半乳糖苷酶活性，并且是乳酸乳球菌IL1403中参与乳糖水解的主要酶。