Glykeria-Myrto Anagnostou, Theodora Skarlatoudi, Vasileios Theodorakis, Loulouda Bosnea, Marios Mataragas
Department of Dairy Research, Institute of Technology of Agricultural Products, Hellenic Agricultural Organization "DIMITRA", Katsikas, 45221 Ioannina, Epirus, Greece.
General Agricultural Cooperative of Ioannina "Farmers' Union", Krya, 45500 Ioannina, Epirus, Greece.
Vet Sci. 2025 Jul 23;12(8):685. doi: 10.3390/vetsci12080685.
The role of spp. in food is debated since this group of lactic acid bacteria contains opportunistic pathogenic strains, some of which exhibit a multidrug-resistant profile. In livestock farms, the use of antibiotics is the most common practice to deal with mastitis-causing bacteria. However, the heavy usage and/or misuse of antibiotics has led to the emergence of antibiotic resistance. This study aimed to genetically and phenotypically characterize strains isolated from raw sheep milk. Samples were collected over one year from the bulk tank of a dairy sheep farm and cultured on selective media. Isolates were purified and analyzed by whole-genome sequencing and antimicrobial susceptibility testing. The isolates were divided into clusters and the corresponding species were identified along with their genes related to virulence and antibiotic resistance. The pan-, core- and accessory-genomes of the strains were determined. Finally, the antibiotic-resistant profile of selected strains was examined and associated with their genomic characterization. These findings contribute to a better understanding of epidemiology, providing comprehensive profiles of their virulence and resistance genes. The presence of antibiotic-resistant bacteria in raw sheep milk destined for the production of cheese should raise awareness.
由于这类乳酸菌包含机会致病菌株，其中一些表现出多重耐药性，因此其在食品中的作用存在争议。在畜牧场，使用抗生素是应对引起乳腺炎细菌的最常见做法。然而，抗生素的大量使用和/或滥用导致了抗生素耐药性的出现。本研究旨在从基因和表型上对从生羊乳中分离出的菌株进行表征。在一年的时间里，从一个奶羊场的贮奶罐中采集样本，并在选择性培养基上进行培养。对分离株进行纯化，并通过全基因组测序和抗菌药敏试验进行分析。将分离株分为不同的簇，并鉴定相应的物种以及与其毒力和抗生素耐药性相关的基因。确定了这些菌株的泛基因组、核心基因组和辅助基因组。最后，检测了所选菌株的抗生素耐药谱，并将其与基因组特征相关联。这些发现有助于更好地了解其流行病学，提供其毒力和耐药基因的全面概况。用于生产奶酪的生羊乳中存在抗生素耐药菌应引起关注。
